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Int. J. Mol. Sci. 2017, 18(7), 1460; doi:10.3390/ijms18071460

Impact of Premature Senescence on Radiosensitivity Measured by High Throughput Cell-Based Assays

Department of Oncology, University of Alberta, Cross Cancer Institute, Edmonton, AB T6G 1Z2, Canada
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Received: 11 June 2017 / Revised: 27 June 2017 / Accepted: 1 July 2017 / Published: 6 July 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

In most p53 wild-type human cell types, radiosensitivity evaluated by the colony formation assay predominantly reflects stress-induced premature senescence (SIPS) and not cell death (Int. J. Mol. Sci. 2017, 18, 928). SIPS is a growth-arrested state in which the cells acquire flattened and enlarged morphology, remain viable, secrete growth-promoting factors, and can give rise to tumor-repopulating progeny. The impact of SIPS on radiosensitivity measured by short-term assays remains largely unknown. We report that in four p53 wild-type human solid tumor-derived cell lines (HCT116, SKNSH, MCF7 and A172): (i) the conventional short-term growth inhibition assay (3 days post-irradiation) generates radiosensitivity data comparable to that measured by the laborious and time-consuming colony formation assay; (ii) radiation dose-response curves obtained by multiwell plate colorimetric/fluorimetric assays are markedly skewed towards radioresistance, presumably reflecting the emergence of highly enlarged, growth-arrested and viable cells; and (iii) radiation exposure (e.g., 8 Gy) does not trigger apoptosis or loss of viability over a period of 3 days post-irradiation. Irrespective of the cell-based assay employed, caution should be exercised to avoid misinterpreting radiosensitivity data in terms of loss of viability and, hence, cell death. View Full-Text
Keywords: ionizing radiation; p53 signaling; premature senescence; viability; proliferation; apoptosis; colony forming ability; MTT; XTT; CellTiter-Blue ionizing radiation; p53 signaling; premature senescence; viability; proliferation; apoptosis; colony forming ability; MTT; XTT; CellTiter-Blue
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Mirzayans, R.; Andrais, B.; Murray, D. Impact of Premature Senescence on Radiosensitivity Measured by High Throughput Cell-Based Assays. Int. J. Mol. Sci. 2017, 18, 1460.

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