Next Article in Journal
Gene Therapy for Pancreatic Cancer: Specificity, Issues and Hopes
Next Article in Special Issue
Hypotheses on the Potential of Rice Bran Intake to Prevent Gastrointestinal Cancer through the Modulation of Oxidative Stress
Previous Article in Journal
BrWRKY65, a WRKY Transcription Factor, Is Involved in Regulating Three Leaf Senescence-Associated Genes in Chinese Flowering Cabbage
Previous Article in Special Issue
Therapeutic Applications of Rose Hips from Different Rosa Species
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(6), 1229; doi:10.3390/ijms18061229

The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection

1
Department of Pharmacology, Qingdao University Medical College, 308 Ning Xia Road, Qingdao 266071, China
2
Qingdao Municipal Center for Disease Control & Prevention, 175 Shandong Road, Qingdao 266071, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: José L. Quiles and Lorenza Speranza
Received: 13 May 2017 / Revised: 27 May 2017 / Accepted: 5 June 2017 / Published: 8 June 2017
(This article belongs to the Special Issue Correlation between Nutrition, Oxidative Stress and Disease)
View Full-Text   |   Download PDF [5354 KB, uploaded 8 June 2017]   |  

Abstract

Perfluorooctanoic acid (PFOA) is an environmental contaminant that could induce developmental cardiotoxicity in a chicken embryo, which may be alleviated by l-carnitine. To explore the roles of reactive oxygen species (ROS) and nitric oxide (NO) in such changes and the potential effects of l-carnitine, fertile chicken eggs were exposed to PFOA via an air cell injection, with or without l-carnitine co-treatment. The ROS and NO levels in chicken embryo hearts were determined with electron spin resonance (ESR), and the protein levels of the nuclear factor κ-light chain-enhancer of activated B cells (NF-κB) p65 and inducible nitric oxide synthase (iNOS) in chicken embryo hearts were assessed with western blotting. The results of ESR indicated that PFOA exposure induced an elevation in the ROS levels in ED19 chicken embryo hearts and hatchling chicken hearts, while l-carnitine could alleviate such changes. Meanwhile, increased NO levels were observed in ED19 embryo hearts and hatchling hearts following PFOA exposure, while l-carnitine co-treatment exerted modulatory effects. Western blotting revealed that p65 translocation in ED19 embryo hearts and hatchling hearts was enhanced by PFOA, while l-carnitine co-treatment alleviated such changes. iNOS expression levels in ED19 embryo hearts followed the same pattern as NO levels, while a suppression of expression was observed in hatchling hearts exposed to PFOA. ROS/NF-κB p65 and iNOS/NO seem to be involved in the late stage (ED19 and post hatch) of PFOA-induced developmental cardiotoxicity in a chicken embryo. l-carnitine could exert anti-oxidant and NO modulatory effects in the developing chicken embryo hearts, which likely contribute to its cardioprotective effects. View Full-Text
Keywords: perfluorooctanoic acid-induced developmental cardiotoxicity; reactive oxygen species; nitric oxide; l-carnitine; electron spin resonance; p65; iNOS perfluorooctanoic acid-induced developmental cardiotoxicity; reactive oxygen species; nitric oxide; l-carnitine; electron spin resonance; p65; iNOS
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhao, M.; Jiang, Q.; Wang, W.; Geng, M.; Wang, M.; Han, Y.; Wang, C. The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection. Int. J. Mol. Sci. 2017, 18, 1229.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top