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Int. J. Mol. Sci. 2017, 18(6), 1162; doi:10.3390/ijms18061162

TRAV7-2*02 Expressing CD8+ T Cells Are Responsible for Palladium Allergy

1
Department of Immunobiology, Institute of Development, Aging and Cancer, Tohoku University 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan
2
Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
3
Department of Pharmacology, School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomitamachi, Koriyama, Fukushima 963-8611, Japan
4
Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aza Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Reinhard Dallinger
Received: 7 March 2017 / Revised: 24 May 2017 / Accepted: 26 May 2017 / Published: 31 May 2017
(This article belongs to the Special Issue Metal Metabolism in Animals II)
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Abstract

While metallic biomaterials have led to an improvement in the quality of life, metal allergies, especially to palladium (Pd), has caused a recent increase in allergic patients. Metal allergy is known to be a T cell-mediated delayed-type hypersensitivity (DTH); however, the pathogenic T cell subsets and the specific T cell receptor (TCR) have not been identified. Therefore, we attempted to identify the pathogenic T cells responsible for Pd allergy. We found that activating CD8+ T cells significantly increased and that the TRAV (TCRα variable) 7-2*02 chain skewed in Pd allergic mice. Furthermore, adoptive transfer experiments revealed that in vitro-cultured Pd-stimulated antigen presenting cells (APCs) function as memory APCs with recipient mice developing Pd allergy and that the frequency of TRAV7-2*02 increases the same as conventional Pd allergic mice. In contrast, neither proliferation of CD8+ T cells nor increasing of TRAV7-2*02 was observed in major histocompatibility complex I (MHC I)-deficient Pd-APCs transferred to mice. Taken together, we revealed that TRAV7-2*02-expressing CD8+ T cells are the pathogenic T cells for the development of Pd allergy. We also identified the CDR3 consensus motif of pathogenic TCRs as CAAXSGSWQLIF in TRAV7-2*02/TRAJ (TCRα junction)22*01 positive cells. These results suggest that the specific TCRs represent novel targets for the development of diagnostics and treatments for metal allergy. View Full-Text
Keywords: animal models; autoimmunity; T cells; biomaterial(s); metal allergy; T cell receptor (TCR) animal models; autoimmunity; T cells; biomaterial(s); metal allergy; T cell receptor (TCR)
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MDPI and ACS Style

Takeda, Y.; Suto, Y.; Ito, K.; Hashimoto, W.; Nishiya, T.; Ueda, K.; Narushima, T.; Takahashi, T.; Ogasawara, K. TRAV7-2*02 Expressing CD8+ T Cells Are Responsible for Palladium Allergy. Int. J. Mol. Sci. 2017, 18, 1162.

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