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Open AccessCommunication
Int. J. Mol. Sci. 2017, 18(6), 1139; doi:10.3390/ijms18061139

Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties

1
Institut für Organische Chemie, Universität Regensburg, Universitätsstr. 31, Regensburg 93053, Germany
2
Institute of Advanced Chemistry of Catalonia-Spanish National Research Council (IQAC-CSIC), Jordi Girona 18-26, Barcelona 08034, Spain
3
Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Jordi Girona 18-26, Barcelona 08034, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Andreas Taubert
Received: 15 March 2017 / Revised: 9 May 2017 / Accepted: 22 May 2017 / Published: 26 May 2017
(This article belongs to the Section Biomaterial Sciences)
View Full-Text   |   Download PDF [1263 KB, uploaded 26 May 2017]   |  

Abstract

Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antisense oligonucleotide (Luc) and non-ionic surfactant polysorbate 80, were investigated regarding their ζ potential, cytotoxicity and transfection efficiency. Deoxyribonucleic acid- (DNA) forming complexes in the presence of cationic vesicles were also investigated in terms of small-angle X-ray scattering (SAXS). The studied cationic vesicles showed very little, if any, toxicity against HeLa cells. Transfection abilities proved to vary strongly depending on the present quaternary ammonium moiety. View Full-Text
Keywords: antisense oligonucleotide; cationic polymers; cationic vesicles; ionenes; transfection; polycations; polyplexes; quaternary ammonium antisense oligonucleotide; cationic polymers; cationic vesicles; ionenes; transfection; polycations; polyplexes; quaternary ammonium
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mayr, J.; Grijalvo, S.; Bachl, J.; Pons, R.; Eritja, R.; Díaz Díaz, D. Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties. Int. J. Mol. Sci. 2017, 18, 1139.

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