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Int. J. Mol. Sci. 2017, 18(5), 887; doi:10.3390/ijms18050887

Integrative RNA- and miRNA-Profile Analysis Reveals a Likely Role of BR and Auxin Signaling in Branch Angle Regulation of B. napus

1
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
2
John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 9 February 2017 / Accepted: 18 April 2017 / Published: 8 May 2017
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [4325 KB, uploaded 8 May 2017]   |  

Abstract

Oilseed rape (Brassica napus L.) is the second largest oilseed crop worldwide and one of the most important oil crops in China. As a component of plant architecture, branch angle plays an important role in yield performance, especially under high-density planting conditions. However, the mechanisms underlying the regulation of branch angle are still largely not understood. Two oilseed rape lines with significantly different branch angles were used to conduct RNA- and miRNA-profiling at two developmental stages, identifying differential expression of a large number of genes involved in auxin- and brassinosteroid (BR)-related pathways. Many auxin response genes, including AUX1, IAA, GH3, and ARF, were enriched in the compact line. However, a number of genes involved in BR signaling transduction and biosynthesis were down-regulated. Differentially expressed miRNAs included those involved in auxin signaling transduction. Expression patterns of most target genes were fine-tuned by related miRNAs, such as miR156, miR172, and miR319. Some miRNAs were found to be differentially expressed at both developmental stages, including three miR827 members. Our results provide insight that auxin- and BR-signaling may play a pivotal role in branch angle regulation. View Full-Text
Keywords: miRNA; branch angle; Brassica napus; deep sequencing; auxin; brassinosteroid miRNA; branch angle; Brassica napus; deep sequencing; auxin; brassinosteroid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cheng, H.; Hao, M.; Wang, W.; Mei, D.; Wells, R.; Liu, J.; Wang, H.; Sang, S.; Tang, M.; Zhou, R.; Chu, W.; Fu, L.; Hu, Q. Integrative RNA- and miRNA-Profile Analysis Reveals a Likely Role of BR and Auxin Signaling in Branch Angle Regulation of B. napus. Int. J. Mol. Sci. 2017, 18, 887.

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