Thioredoxin-Interacting Protein Mediates Apoptosis in Early Brain Injury after Subarachnoid Haemorrhage
Abstract
:1. Introduction
2. Results
2.1. TXNIP Expression Increased Obviously after SAH
2.2. TXNIP Was Expressed in Neurons and Is Colocalized with TUNEL Positive Cells
2.3. Downregulation of TXNIP by Resveratrol (RES) and siRNA
2.4. Downregulation of TXNIP by PERK Inhibition
2.5. SAH Grade and Mortality
2.6. Neurological Deficits
2.7. BBB Permeability
2.8. Brain Water Content
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Endovascular Perforation Model of SAH
4.3. Drug Administration
4.3.1. Resveratrol and TXNIP siRNA Injection
4.3.2. GSK2656157 Injection
4.4. Neurological Scores
4.5. SAH Grade and Mortality
4.6. Blood-Brain Barrier (BBB) Permeability
4.7. Brain Water Content
4.8. Immunofluorescence
4.9. TUNEL Staining
4.10. Western Blot
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhao, Q.; Che, X.; Zhang, H.; Tan, G.; Liu, L.; Jiang, D.; Zhao, J.; Xiang, X.; Sun, X.; He, Z. Thioredoxin-Interacting Protein Mediates Apoptosis in Early Brain Injury after Subarachnoid Haemorrhage. Int. J. Mol. Sci. 2017, 18, 854. https://doi.org/10.3390/ijms18040854
Zhao Q, Che X, Zhang H, Tan G, Liu L, Jiang D, Zhao J, Xiang X, Sun X, He Z. Thioredoxin-Interacting Protein Mediates Apoptosis in Early Brain Injury after Subarachnoid Haemorrhage. International Journal of Molecular Sciences. 2017; 18(4):854. https://doi.org/10.3390/ijms18040854
Chicago/Turabian StyleZhao, Qing, Xudong Che, Hongxia Zhang, Guanping Tan, Liu Liu, Dengzhi Jiang, Jun Zhao, Xiang Xiang, Xiaochuan Sun, and Zhaohui He. 2017. "Thioredoxin-Interacting Protein Mediates Apoptosis in Early Brain Injury after Subarachnoid Haemorrhage" International Journal of Molecular Sciences 18, no. 4: 854. https://doi.org/10.3390/ijms18040854