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Int. J. Mol. Sci. 2017, 18(4), 846; doi:10.3390/ijms18040846

The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function

1
Department of Biomedical Engineering, Mokwon University, Daejeon 35349, Korea
2
Department of Formula Sciences, College of Korean Medicine, Dongeui University, Busan 47340, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Marica Bakovic
Received: 3 March 2017 / Revised: 31 March 2017 / Accepted: 12 April 2017 / Published: 17 April 2017
(This article belongs to the Special Issue Nutrigenomics of Risk Factors for Disease)
View Full-Text   |   Download PDF [3831 KB, uploaded 18 April 2017]   |  

Abstract

Similar to neoplastic tissues, growth and development of adipose tissue are thought to be angiogenesis-dependent. Since visceral adipose tissue (VAT) is associated with development and progression of nonalcoholic fatty liver disease (NAFLD), we hypothesized that angiogenesis inhibition would attenuate obesity-induced NAFLD. We fed C57BL/6J mice a low-fat diet (LFD, chow 10% kcal fat), a high-fat diet (HFD, 45% kcal fat) or HFD supplemented with the lemon-balm extract ALS-L1023 (HFD-ALS) for 15 weeks. ALS-L1023 reduced endothelial cell-tube formation in vitro. HFD increased VAT angiogenesis and induced weight gains including body weight, VAT mass and visceral adipocyte size compared with LFD. However, HFD-ALS led to weight reductions without affecting calorie intake compared with HFD. HFD-ALS also reduced serum ALT and AST levels and improved lipid metabolism. HFD-ALS suppressed steatosis, infiltration of inflammatory cells, and accumulation of collagen in livers. HFD-ALS modulated hepatic expression of genes involved in lipid metabolism, inflammation, fibrosis, antioxidation, and apoptosis. Concomitantly, analysis of VAT function revealed that HFD-ALS led to fewer CD68-positive macrophage numbers and lower expression of inflammatory cytokines compared with HFD. Our findings show that the anti-angiogenic herbal extract ALS-L1023 attenuates NAFLD by targeting VAT during obesity, suggesting that angiogenesis inhibitors could aid in the treatment and prevention of obesity-induced human NAFLD. View Full-Text
Keywords: herbal medicine; Melissa officinalis; visceral adipose inflammation; visceral obesity herbal medicine; Melissa officinalis; visceral adipose inflammation; visceral obesity
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MDPI and ACS Style

Kim, J.; Lee, H.; Lim, J.; Oh, J.; Shin, S.S.; Yoon, M. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function. Int. J. Mol. Sci. 2017, 18, 846.

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