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Int. J. Mol. Sci. 2017, 18(4), 751; doi:10.3390/ijms18040751

Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing

1
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2
College of Informatics, Huazhong Agricultural University, Wuhan 430070, China
3
College of Agricultural Animal Husbandry, Tibet University, Linzhi 850012, China
4
State Key Laboratory of Agricultural Microbiology, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
5
Senior Scientific Officer, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 13 January 2017 / Revised: 3 March 2017 / Accepted: 24 March 2017 / Published: 1 April 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [3221 KB, uploaded 1 April 2017]   |  

Abstract

Gastrointestinal nematodes (GINs) are one of the most economically important parasites of small ruminants and a major animal health concern in many regions of the world. However, the molecular mechanisms of the host response to GIN infections in goat are still little known. In this study, two genetically distinct goat populations, one relatively resistant and the other susceptible to GIN infections, were identified in Yichang goat and then four individuals in each group were chosen to compare mRNA expression profiles using RNA-seq. Field experiment showed lower worm burden, delayed and reduced egg production in the relatively resistant group than the susceptible group. The analysis of RNA-seq showed that 2369 genes, 1407 of which were up-regulated and 962 down-regulated, were significantly (p < 0.001) differentially expressed between these two groups. Functional annotation of the 298 genes more highly expressed in the resistant group yielded a total of 46 significant (p < 0.05) functional annotation clusters including 31 genes (9 in innate immunity, 13 in immunity, and 9 in innate immune response) related to immune biosynthetic process as well as transforming growth factor (TGF)-β, mitogen-activated protein kinase (MAPK), and cell adhesion molecules (CAMs) pathways. Our findings provide insights that are immediately relevant for the improvement of host resistance to GIN infections and which will make it possible to know the mechanisms underlying the resistance of goats to GIN infections. View Full-Text
Keywords: transcriptome; genetic resistant; gastrointestinal nematodes; RNA-seq; goat transcriptome; genetic resistant; gastrointestinal nematodes; RNA-seq; goat
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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    Doi: NCBI, Sequence Read Archive (SRA) (Bio project ID: PRJNA 341785, accession numbers SRX2181195, SRX2181163, SRX2181139, SRX2181120, SRX2181119, SRX2181118, SRX2181117 and SRX2181116).
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MDPI and ACS Style

Bhuiyan, A.A.; Li, J.; Wu, Z.; Ni, P.; Adetula, A.A.; Wang, H.; Zhang, C.; Tang, X.; Bhuyan, A.A.; Zhao, S.; Du, X. Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing. Int. J. Mol. Sci. 2017, 18, 751.

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