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Int. J. Mol. Sci. 2017, 18(4), 743; doi:10.3390/ijms18040743

Early Assessment of Colorectal Cancer by Quantifying Circulating Tumor Cells in Peripheral Blood: ECT2 in Diagnosis of Colorectal Cancer

1
Department of Surgical Pathology, Changhua Christian Hospital, Changhua 8864, Taiwan
2
Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 88637, Taiwan
3
School of Medicine, Chung Shan Medical University, Taichung 8864, Taiwan
4
Department of Urology, Chung Shan Medical University Hospital, Taichung 8864, Taiwan
5
Institute of Medicine, Chung Shan Medical University, Taichung 8864, Taiwan
6
Department of Medical Education, Chung Shan Medical University Hospital, Taichung 8864, Taiwan
7
Department of Colon and Rectal Surgery, Changhua Christian Hospital, Changhua, Taiwan
8
Institute of Biomedical Sciences, Academia Sinica, Taipei 8862, Taiwan
9
Institute of Medicine, Chung Shan Medical University, Taichuang 8864, Taiwan
The author, Konan Peck, was deceased during study.
*
Authors to whom correspondence should be addressed.
Received: 13 January 2017 / Revised: 13 March 2017 / Accepted: 24 March 2017 / Published: 31 March 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [1517 KB, uploaded 4 April 2017]   |  

Abstract

Circulating tumor cells (CTCs) in peripheral blood is an indication of poor prognosis for patients with different cancer types. However, most of the available technologies for detecting CTCs show low sensitivity and specificity. Therefore, we attempted to find an alternative marker for CTCs of colorectal cancer. We have directly extracted RNA from CTCs contained in 1.5 mL peripheral blood from 90 colorectal cancer patients and 151 healthy donors, and screened these samples for candidate marker genes by nested real-time quantitative polymerase chain reaction (PCR). From genes selected from a public database of microarray analyses, we successfully identified epithelial cell transforming sequence 2 oncogene (ECT2) as a gene that exhibits high differential expression ratios (p < 0.01). ECT2 displays good sensitivity and specificity, with an area under the curve (AUC) value of 0.821. This marker gene also has a high detection rate in patients with serum carcinoembryonic antigen (CEA) concentrations below the diagnostic threshold of 5 ng/mL. The expression of ECT2 can therefore serve as an alternative measurement that can compensate for the inadequacy of the current CEA test in the diagnosis and monitoring of colorectal cancer patients. View Full-Text
Keywords: circulating tumor cell; ECT2; CEA; colorectal cancer; biomarker circulating tumor cell; ECT2; CEA; colorectal cancer; biomarker
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MDPI and ACS Style

Chen, C.-J.; Sung, W.-W.; Chen, H.-C.; Chern, Y.-J.; Hsu, H.-T.; Lin, Y.-M.; Lin, S.-H.; Peck, K.; Yeh, K.-T. Early Assessment of Colorectal Cancer by Quantifying Circulating Tumor Cells in Peripheral Blood: ECT2 in Diagnosis of Colorectal Cancer. Int. J. Mol. Sci. 2017, 18, 743.

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