Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy
Abstract
:1. Introduction
2. Results
2.1. Expression of Chemokines and Their Receptors in the Bovine Endometrium during Early Pregnancy
2.2. Effects of IFNT and FMP on Chemokine Expression in Cultured Endometrial Tissues
2.3. Effects of Chemokines, IFNT, FMP, TNF, and LIF on ISG15, MX1, COX2, OTR, and ESR1 Expression in Cultured Endometrial Tissues
3. Discussion
4. Materials and Methods
4.1. Collection of the Bovine Uterus and Fetal Trophoblast
4.2. Microarray Analysis
4.3. Real-Time PCR
4.4. Immunohistochemistry
4.5. Endometrial Tissue Culture
4.6. Statistical Analyses
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
IFNT | interferon τ |
COX2 | cyclooxygenase 2 |
OTR | oxytocin receptor |
ESR1 | estrogen receptor α |
CL | corpus luteum |
PG | prostaglandin |
TNF | tumor necrosis factor α |
LIF | leukemia inhibitory factor |
FMP | supernatant derived from homogenized fetal trophoblast |
MCP | monocyte chemoattractant protein |
MIP | macrophage inflammatory protein |
E2 | estradiol-17β |
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Genes | Day 15 (344 Genes) | Day 18 (1336 Genes) |
---|---|---|
CCL2 (MCP-1) | 3.58 | - |
CCL8 (MCP-2) | 4.91 | 12.9 |
CCL11 (Eotaxin) | - | 61.1 |
CCL14 (HCC-1) | 2.67 | - |
CCL16 (HCC-4) | 2.55 | - |
CXCL10 (IP-10) | 2.10 | 21.1 |
Genes | Sequence (5′-3′) | GenBank Accession Number | Size (bp) |
---|---|---|---|
CCL2 | Forward: TGCAGACCCCAAGCAGAAAT | NM_174006 | 144 |
Reverse: AGAGGGCAGTTAGGGAAAGC | |||
CCL8 | Forward: AACATGAAGGTCTCCGCTGG | NM_174007 | 108 |
Reverse: GCAGCAGGTGATTGGGGTAG | |||
CCL11 | Forward: TCACGAGCAGCAAATGTCCT | NM_205773 | 101 |
Reverse: CATGGCATTCTGGACCCACT | |||
CCL14 | Forward: ACTAAATTTCCCCGCTCGCT | NM_001046585 | 121 |
Reverse: TGGCCAAACTTCTGCAGAGT | |||
CCL16 | Forward: GCCCACTGAGAGGATGAAGG | XM_002695627 | 129 |
Reverse: TACTTCAGGCAGCAGTTGGG | |||
CXCL10 | Forward: CTCGAACACGGAAAGAGGCA | NM_001046551 | 117 |
Reverse: TCCACGGACAATTAGGGCTT | |||
ISG15 | Forward: GCAGACCAGTTCTGGCTGTCT | NM_174366 | 58 |
Reverse: CCAGCGGGTGCTCATCAT | |||
MX1 | Forward: GAGGTGGACCCCCAAGGA | NM_173940 | 58 |
Reverse: CCACCAGATCGGGCTTTGT | |||
COX2 | Forward: TGTGAAAGGGAGGAAAGAGC | AF004944 | 115 |
Reverse: GGCAAAGAATGCAAACATCA | |||
OTR | Forward: TGTGCTGGACGCCATTCTT | NM_174134 | 93 |
Reverse: GGAGCATGGCGATGATGAAAG | |||
ESR1 | Forward: CAGGCACATGAGCAACAAAG | NM_001001443 | 84 |
Reverse: TCCAGCAGCAGGTCGTAGAG | |||
SUZ12 | Forward: GAACACCTATCACACACATTCTTGT | NM_001205587 | 130 |
Reverse: TAGAGGCGGTTGTGTCCACT |
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Sakumoto, R.; Hayashi, K.-G.; Fujii, S.; Kanahara, H.; Hosoe, M.; Furusawa, T.; Kizaki, K. Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy. Int. J. Mol. Sci. 2017, 18, 742. https://doi.org/10.3390/ijms18040742
Sakumoto R, Hayashi K-G, Fujii S, Kanahara H, Hosoe M, Furusawa T, Kizaki K. Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy. International Journal of Molecular Sciences. 2017; 18(4):742. https://doi.org/10.3390/ijms18040742
Chicago/Turabian StyleSakumoto, Ryosuke, Ken-Go Hayashi, Shiori Fujii, Hiroko Kanahara, Misa Hosoe, Tadashi Furusawa, and Keiichiro Kizaki. 2017. "Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy" International Journal of Molecular Sciences 18, no. 4: 742. https://doi.org/10.3390/ijms18040742