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Int. J. Mol. Sci. 2017, 18(3), 678; doi:10.3390/ijms18030678

Cytotoxicity, Bactericidal, and Antioxidant Activity of Sodium Alginate Hydrosols Treated with Direct Electric Current

1
Department of Animal Products Technology and Quality Management, The Faculty of Food Science, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland
2
Department of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland
3
Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Antonella Piozzi and Iolanda Francolini
Received: 23 January 2017 / Revised: 14 March 2017 / Accepted: 17 March 2017 / Published: 22 March 2017
(This article belongs to the Section Biomaterial Sciences)
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Abstract

The aim of the study was to investigate the effect of using direct electric current (DC) of 0, 200, and 400 mA for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. The pH, oxidation-reduction potential (ORP), electrical conductivity (EC), and available chlorine concentration (ACC) were measured. The effect of sodium alginate hydrosols treated with DC on Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Micrococcus luteus, Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica, Pseudomonas fluorescence, and RAW 264.7 and L929 cells was investigated. Subsequently, the antioxidant properties of hydrosols were evaluated by determining the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) and ferric reducing antioxidant power (FRAP). The results have shown that after applying 400 mA in hydrosol samples with 0.1% and 0.2% NaCl all tested bacteria were inactivated. The ACC concentration of C400 samples with NaCl was equal to 13.95 and 19.71 mg/L, respectively. The cytotoxicity analysis revealed that optimized electric field conditions and the addition of sodium chloride allow for the avoidance of toxicity effects on normal cells without disturbing the antibacterial effects. Due to the presence of oxidizing substances, the DPPH of variants treated with DC was lower than the DPPH of control samples. View Full-Text
Keywords: sodium alginate; hydrosol; direct electric current; antibacterial activity; cytotoxicity; pH; STEM; DPPH; FRAP sodium alginate; hydrosol; direct electric current; antibacterial activity; cytotoxicity; pH; STEM; DPPH; FRAP
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MDPI and ACS Style

Król, Ż.; Marycz, K.; Kulig, D.; Marędziak, M.; Jarmoluk, A. Cytotoxicity, Bactericidal, and Antioxidant Activity of Sodium Alginate Hydrosols Treated with Direct Electric Current. Int. J. Mol. Sci. 2017, 18, 678.

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