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Int. J. Mol. Sci. 2017, 18(3), 566; doi:10.3390/ijms18030566

A Human Antibody That Binds to the Sixth Ig-Like Domain of VCAM-1 Blocks Lung Cancer Cell Migration In Vitro

1
Research Center, Scripps Korea Antibody Institute, Chuncheon 24341, Korea
2
Specific Organs Cancer Branch, Research Institute, National Cancer Center, Goyang 10408, Korea
3
Department of Biochemistry and Molecular Biology, Seoul National University, Seoul 03087, Korea
4
Departments of Bioinspired Science and Life Science, Ewha Womans University, Seoul 03760, Korea
5
Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), Daejeon 34132, Korea
6
Department of Integrated OMICS for Biomedical Science, College of Pharmacy, Yonsei University, Incheon 21983, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Hsueh-Wei Chang
Received: 17 January 2017 / Revised: 27 February 2017 / Accepted: 3 March 2017 / Published: 6 March 2017
(This article belongs to the Special Issue Tumor Targeting Therapy and Selective Killing)
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Abstract

Vascular cell adhesion molecule-1 (VCAM-1) is closely associated with tumor progression and metastasis. However, the relevance and role of VCAM-1 in lung cancer have not been clearly elucidated. In this study, we found that VCAM-1 was highly overexpressed in lung cancer tissue compared with that of normal lung tissue, and high VCAM-1 expression correlated with poor survival in lung cancer patients. VCAM-1 knockdown reduced migration of A549 human lung cancer cells into Matrigel, and competitive blocking experiments targeting the Ig-like domain 6 of VCAM-1 (VCAM-1-D6) demonstrated that the VCAM-1-D6 domain was critical for VCAM-1 mediated A549 cell migration into Matrigel. Next, we developed a human monoclonal antibody specific to human and mouse VCAM-1-D6 (VCAM-1-D6 huMab), which was isolated from a human synthetic antibody library using phage display technology. Finally, we showed that VCAM-1-D6 huMab had a nanomolar affinity for VCAM-1-D6 and that it potently suppressed the migration of A549 and NCI-H1299 lung cancer cell lines into Matrigel. Taken together, these results suggest that VCAM-1-D6 is a key domain for regulating VCAM-1-mediated lung cancer invasion and that our newly developed VCAM-1-D6 huMab will be a useful tool for inhibiting VCAM-1-expressing lung cancer cell invasion. View Full-Text
Keywords: human antibody; invasion; lung cancer; Matrigel; migration; VCAM-1; VCAM-1-D6 human antibody; invasion; lung cancer; Matrigel; migration; VCAM-1; VCAM-1-D6
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MDPI and ACS Style

Kim, M.R.; Jang, J.H.; Park, C.S.; Kim, T.-K.; Kim, Y.-J.; Chung, J.; Shim, H.; Nam, I.H.; Han, J.M.; Lee, S. A Human Antibody That Binds to the Sixth Ig-Like Domain of VCAM-1 Blocks Lung Cancer Cell Migration In Vitro. Int. J. Mol. Sci. 2017, 18, 566.

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