Ovalbumin with Glycated Carboxyl Groups Shows Membrane-Damaging Activity
AbstractThe aim of the present study was to investigate whether glycated ovalbumin (OVA) showed novel activity at the lipid-water interface. Mannosylated OVA (Man-OVA) was prepared by modification of the carboxyl groups with p-aminophenyl α-dextro (d)-mannopyranoside. An increase in the number of modified carboxyl groups increased the membrane-damaging activity of Man-OVA on cell membrane-mimicking vesicles, whereas OVA did not induce membrane permeability in the tested phospholipid vesicles. The glycation of carboxyl groups caused a notable change in the gross conformation of OVA. Moreover, owing to their spatial positions, the Trp residues in Man-OVA were more exposed, unlike those in OVA. Fluorescence quenching studies suggested that the Trp residues in Man-OVA were located on the interface binds with the lipid vesicles, and their microenvironment was abundant in positively charged residues. Although OVA and Man-OVA showed a similar binding affinity for lipid vesicles, the lipid-interacting feature of Man-OVA was distinct from that of OVA. Chemical modification studies revealed that Lys and Arg residues, but not Trp residues, played a crucial role in the membrane-damaging activity of Man-OVA. Taken together, our data suggest that glycation of carboxyl groups causes changes in the structural properties and membrane-interacting features of OVA, generating OVA with membrane-perturbing activities at the lipid-water interface. View Full-Text
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Tang, C.-C.; Shi, Y.-J.; Chen, Y.-J.; Chang, L.-S. Ovalbumin with Glycated Carboxyl Groups Shows Membrane-Damaging Activity. Int. J. Mol. Sci. 2017, 18, 520.
Tang C-C, Shi Y-J, Chen Y-J, Chang L-S. Ovalbumin with Glycated Carboxyl Groups Shows Membrane-Damaging Activity. International Journal of Molecular Sciences. 2017; 18(3):520.Chicago/Turabian Style
Tang, Ching-Chia; Shi, Yi-Jun; Chen, Ying-Jung; Chang, Long-Sen. 2017. "Ovalbumin with Glycated Carboxyl Groups Shows Membrane-Damaging Activity." Int. J. Mol. Sci. 18, no. 3: 520.
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