Effects of Remote Ischemic Preconditioning on Heme Oxygenase-1 Expression and Cutaneous Wound Repair
AbstractSkin wounds may lead to scar formation and impaired functionality. Remote ischemic preconditioning (RIPC) can induce the anti-inflammatory enzyme heme oxygenase-1 (HO-1) and protect against tissue injury. We aim to improve cutaneous wound repair by RIPC treatment via induction of HO-1. RIPC was applied to HO-1-luc transgenic mice and HO-1 promoter activity and mRNA expression in skin and several other organs were determined in real-time. In parallel, RIPC was applied directly or 24h prior to excisional wounding in mice to investigate the early and late protective effects of RIPC on cutaneous wound repair, respectively. HO-1 promoter activity was significantly induced on the dorsal side and locally in the kidneys following RIPC treatment. Next, we investigated the origin of this RIPC-induced HO-1 promoter activity and demonstrated increased mRNA in the ligated muscle, heart and kidneys, but not in the skin. RIPC did not change HO-1 mRNA and protein levels in the wound 7 days after cutaneous injury. Both early and late RIPC did not accelerate wound closure nor affect collagen deposition. RIPC induces HO-1 expression in several organs, but not the skin, and did not improve excisional wound repair, suggesting that the skin is insensitive to RIPC-mediated protection. View Full-Text
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Cremers, N.A.J.; Wever, K.E.; Wong, R.J.; van Rheden, R.E.M.; Vermeij, E.A.; van Dam, G.M.; Carels, C.E.; Lundvig, D.M.S.; Wagener, F.A.D.T.G. Effects of Remote Ischemic Preconditioning on Heme Oxygenase-1 Expression and Cutaneous Wound Repair. Int. J. Mol. Sci. 2017, 18, 438.
Cremers NAJ, Wever KE, Wong RJ, van Rheden REM, Vermeij EA, van Dam GM, Carels CE, Lundvig DMS, Wagener FADTG. Effects of Remote Ischemic Preconditioning on Heme Oxygenase-1 Expression and Cutaneous Wound Repair. International Journal of Molecular Sciences. 2017; 18(2):438.Chicago/Turabian Style
Cremers, Niels A.J.; Wever, Kimberley E.; Wong, Ronald J.; van Rheden, René E.M.; Vermeij, Eline A.; van Dam, Gooitzen M.; Carels, Carine E.; Lundvig, Ditte M.S.; Wagener, Frank A.D.T.G. 2017. "Effects of Remote Ischemic Preconditioning on Heme Oxygenase-1 Expression and Cutaneous Wound Repair." Int. J. Mol. Sci. 18, no. 2: 438.
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