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Int. J. Mol. Sci. 2017, 18(2), 357; doi:10.3390/ijms18020357

Silencing of Two Insulin Receptor Genes Disrupts Nymph-Adult Transition of Alate Brown Citrus Aphid

1
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China
2
Department of Crop Protection, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: T. G. Emyr Davies
Received: 3 January 2017 / Revised: 26 January 2017 / Accepted: 2 February 2017 / Published: 21 February 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Insulin receptors play key roles in growth, development, and polymorphism in insects. Here, we report two insulin receptor genes (AcInR1 and AcInR2) from the brown citrus aphid, Aphis (Toxoptera) citricidus. Transcriptional analyses showed that AcInR1 increased during the nymph–adult transition in alate aphids, while AcInR2 had the highest expression level in second instar nymphs. AcInR1 is important in aphid development from fourth instar nymphs to adults as verified by dsRNA feeding mediated RNAi. The silencing of AcInR1 or/and AcInR2 produced a variety of phenotypes including adults with normal wings, malformed wings, under-developed wings, and aphids failing to develop beyond the nymphal stages. Silencing of AcInR1 or AcInR2 alone, and co-silencing of both genes, resulted in 73% or 60%, and 87% of aphids with problems in the transition from nymph to normal adult. The co-silencing of AcInR1 and AcInR2 resulted in 62% dead nymphs, but no mortality occurred by silencing of AcInR1 or AcInR2 alone. Phenotypes of adults in the dsInR1 and dsInR2 were similar. The results demonstrate that AcInR1 and AcInR2 are essential for successful nymph–adult transition in alate aphids and show that RNAi methods may be useful for the management of this pest. View Full-Text
Keywords: Aphis (Toxoptera) citricidus; insulin receptor genes; RNAi; development Aphis (Toxoptera) citricidus; insulin receptor genes; RNAi; development
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ding, B.-Y.; Shang, F.; Zhang, Q.; Xiong, Y.; Yang, Q.; Niu, J.-Z.; Smagghe, G.; Wang, J.-J. Silencing of Two Insulin Receptor Genes Disrupts Nymph-Adult Transition of Alate Brown Citrus Aphid. Int. J. Mol. Sci. 2017, 18, 357.

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