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Int. J. Mol. Sci. 2017, 18(12), 2699; doi:10.3390/ijms18122699

hASC and DFAT, Multipotent Stem Cells for Regenerative Medicine: A Comparison of Their Potential Differentiation In Vitro

1
Department Clinical Surgical, Diagnostic and Pediatric Sciences, Plastic and Reconstructive Surgery Unit, University of Pavia, 27100 Pavia, Italy
2
Orthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, Italy
3
Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, 27100 Pavia, Italy
4
Department of Clinical Surgical, Diagnostic and Pediatric Sciences, Locomotor System Diseases Unit, University of Pavia, 27100 Pavia, Italy
5
Centre for Health Technologies, University of Pavia, 27100 Pavia, Italy
6
Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, 27100 Pavia, Italy
7
Department of Molecular Medicine, Human Physiology Unit, University of Pavia, 27100 Pavia, Italy
*
Author to whom correspondence should be addressed.
Received: 19 October 2017 / Revised: 23 November 2017 / Accepted: 9 December 2017 / Published: 13 December 2017
(This article belongs to the Special Issue Adipose Stem Cells)
View Full-Text   |   Download PDF [2209 KB, uploaded 13 December 2017]   |  

Abstract

Adipose tissue comprises both adipose and non-adipose cells such as mesenchymal stem cells. These cells show a surface antigenic profile similar to that of bone-marrow-derived MSC. The cells derived from the dedifferentiation of mature adipocytes (DFAT) are another cell population with characteristics of stemness. The aim of this study is to provide evidence of the stemness, proliferation, and differentiation of human adipose stem cells (hASC) and DFAT obtained from human subcutaneous AT and evaluate their potential use in regenerative medicine. Cell populations were studied by histochemical and molecular biology techniques. Both hASC and DFAT were positive for MSC markers. Their proliferative capacity was similar and both populations were able to differentiate into osteogenic, chondrogenic, and adipogenic lineages. DFAT were able to accumulate lipids and their lipoprotein lipase and adiponectin gene expression were high. Alkaline phosphatase and RUNX2 gene expression were greater in hASC than in DFAT at 14 days but became similar after three weeks. Both cell populations were able to differentiate into chondrocytes, showing positive staining with Alcian Blue and gene expression of SOX9 and ACAN. In conclusion, both hASC and DFAT populations derived from AT have a high differentiation capacity and thus may have applications in regenerative medicine. View Full-Text
Keywords: adipocytes; adipose tissue; cell culture in vitro; dedifferentiated fat adipose tissue; fibroblast-like; human adipose stem cells adipocytes; adipose tissue; cell culture in vitro; dedifferentiated fat adipose tissue; fibroblast-like; human adipose stem cells
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MDPI and ACS Style

Saler, M.; Caliogna, L.; Botta, L.; Benazzo, F.; Riva, F.; Gastaldi, G. hASC and DFAT, Multipotent Stem Cells for Regenerative Medicine: A Comparison of Their Potential Differentiation In Vitro. Int. J. Mol. Sci. 2017, 18, 2699.

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