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Int. J. Mol. Sci. 2017, 18(12), 2665; https://doi.org/10.3390/ijms18122665

Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds

1
Department of Molecular Medicine, Human Physiology Unit, University of Pavia, I-27100 Pavia, Italy
2
Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, University of Pavia, I-27100 Pavia, Italy
3
Operative Unit of Orthopaedics and Traumatology, Fondazione IRCCS Policlinico San Matteo, I-27100 Pavia, Italy
4
Department of Earth and Environmental Sciences, University of Pavia, I-27100 Pavia, Italy
*
Author to whom correspondence should be addressed.
Received: 15 November 2017 / Revised: 4 December 2017 / Accepted: 6 December 2017 / Published: 8 December 2017
(This article belongs to the Special Issue Aquaporins: Water Channels Essential for Living Organisms)
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Abstract

Some aquaporins (AQPs) have been recently demonstrated to facilitate the diffusion of hydrogen peroxide (H2O2) from the producing cells to the extracellular fluid, and their reactive oxygen species scavenging properties have been defined. Nevertheless, the identification of different AQPs acting as peroxiporins, their functional role in eustress and distress, and the identification of antioxidant compounds able to regulate AQP gating, remain unsolved. This study aims to investigate, in HeLa cells: (1) the expression of different AQPs; (2) the evaluation of naringenin, quercetin, (R)-aloesaponol III 8-methyl ether, marrubiin, and curcumin antioxidant profiles, via α,α-diphenyl-β-picrylhydrazyl assay; (3) the effect of the compounds on the water permeability in the presence and in the absence of oxidative stress; and (4) the effect of pre- and post-treatment with the compounds on the H2O2 content in heat-stressed cells. Results showed that HeLa cells expressed AQP1, 3, 8, and 11 proteins. The oxidative stress reduced the water transport, and both pre- and post-treatment with the natural compounds recovering the water permeability, with the exception of curcumin. Moreover, the pre- and post-treatment with all the compounds reduced the H2O2 content of heat-stressed cells. This study confirms that oxidative stress reduced water AQP-mediated permeability, reversed by some chemical antioxidant compounds. Moreover, curcumin was shown to regulate AQP gating. This suggests a novel mechanism to regulate cell signaling and survival during stress, and to manipulate key signaling pathways in cancer and degenerative diseases. View Full-Text
Keywords: water channel; oxidative stress; antioxidant compounds; hydrogen peroxide water channel; oxidative stress; antioxidant compounds; hydrogen peroxide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Pellavio, G.; Rui, M.; Caliogna, L.; Martino, E.; Gastaldi, G.; Collina, S.; Laforenza, U. Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds. Int. J. Mol. Sci. 2017, 18, 2665.

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