Next Article in Journal
Adverse Intrauterine Environment and Cardiac miRNA Expression
Next Article in Special Issue
Evaluation of a New Survivin ELISA and UBC® Rapid for the Detection of Bladder Cancer in Urine
Previous Article in Journal
Characterization of the Asiatic Acid Glucosyltransferase, UGT73AH1, Involved in Asiaticoside Biosynthesis in Centella asiatica (L.) Urban
Previous Article in Special Issue
Illumination of a Vision—How Arthur Rimbaud Will Give Us Motivation to Find New Input into Bladder Cancer Biomarker Research
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(12), 2632; doi:10.3390/ijms18122632

Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas

1
Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
2
Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
3
Graduate School of Life Science, Frontier Research Centre for Advanced Material and Life Science, Hokkaido University, Sapporo 060-0810, Japan
4
Department of Social Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
*
Author to whom correspondence should be addressed.
Received: 21 November 2017 / Revised: 1 December 2017 / Accepted: 2 December 2017 / Published: 6 December 2017
View Full-Text   |   Download PDF [2587 KB, uploaded 6 December 2017]   |  

Abstract

The aim of this study to determine whether the aberrant N-glycosylated serum immunoglobulins (Igs) can be applied as a diagnostic marker of urothelial carcinoma (UC). Between 2009 and 2016, we randomly obtained serum available from 237 UC and also 96 prostate cancer as other cancer controls from our serum bank and also obtained—from 339 healthy volunteers (HV)—controls obtained from community-dwelling volunteers in Iwaki Health Promotion Project. A total of 32 types of N-glycan levels on Igs were determined by high-throughput N-glycomics and analyzed by multivariable discriminant analysis. We found five UC-associated aberrant N-glycans changes on Igs and also found that asialo-bisecting GlcNAc type N-glycan on Igs were significantly accumulated in UC patients. The diagnostic N-glycan Score (dNGScore) established by combination of five N-glycans on Igs discriminated UC patients from HV and prostate cancer (PC) patients with 92.8% sensitivity and 97.2% specificity. The area under the curve (AUC) for of the dNGScore was 0.969 for UC detection that was much superior to that of urine cytology (AUC, 0.707) and hematuria (AUC, 0.892). Furthermore, dNGScore can detect hematuria and urine cytology negative patients. The dNGscore based on aberrant N-glycosylation signatures of Igs were found to be promising diagnostic biomarkers of UCs. View Full-Text
Keywords: diagnostic biomarker; urothelial carcinoma of the bladder; upper urinary tract urothelial carcinoma; N-glycomics; immunogloburins; aberrant N-glycosylation diagnostic biomarker; urothelial carcinoma of the bladder; upper urinary tract urothelial carcinoma; N-glycomics; immunogloburins; aberrant N-glycosylation
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tanaka, T.; Yoneyama, T.; Noro, D.; Imanishi, K.; Kojima, Y.; Hatakeyama, S.; Tobisawa, Y.; Mori, K.; Yamamoto, H.; Imai, A.; Yoneyama, T.; Hashimoto, Y.; Koie, T.; Tanaka, M.; Nishimura, S.-I.; Kurauchi, S.; Takahashi, I.; Ohyama, C. Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas. Int. J. Mol. Sci. 2017, 18, 2632.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top