Next Article in Journal
Tunisian Milk Thistle: An Investigation of the Chemical Composition and the Characterization of Its Cold-Pressed Seed Oils
Next Article in Special Issue
Peptide-Mediated Liposome Fusion: The Effect of Anchor Positioning
Previous Article in Journal
Function of microRNAs in the Osteogenic Differentiation and Therapeutic Application of Adipose-Derived Stem Cells (ASCs)
Previous Article in Special Issue
A Membrane-Fusion Model That Exploits a β-to-α Transition in the Hydrophobic Domains of Syntaxin 1A and Synaptobrevin 2
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(12), 2598; https://doi.org/10.3390/ijms18122598

Switching between Successful and Dead-End Intermediates in Membrane Fusion

1
Laboratory of Bioelectrochemistry, A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskiy Prospekt, 119071 Moscow, Russia
2
Department of Theoretical Physics and Quantum Technologies, National University of Science and Technology “MISiS”, 4 Leninskiy Prospekt, 119049 Moscow, Russia
3
Department of Engineering of Technological Equipment, National University of Science and Technology “MISiS”, 4 Leninskiy Prospekt, 119049 Moscow, Russia
4
Laboratory of Electrophysiology, Federal Clinical Center of Physical-Chemical Medicine of FMBA, 1a Malaya Pirogovskaya Street, 119435 Moscow, Russia
5
Department of Physics of Living Systems, Moscow Institute of Physics and Technology (State University), 9 Institutskiy Lane, 141700 Dolgoprudniy Moscow Region, Russia
These authors contributed equally to the work.
*
Author to whom correspondence should be addressed.
Received: 31 October 2017 / Revised: 21 November 2017 / Accepted: 1 December 2017 / Published: 2 December 2017
(This article belongs to the Special Issue Membrane Fusion)
View Full-Text   |   Download PDF [4065 KB, uploaded 2 December 2017]   |  

Abstract

Fusion of cellular membranes during normal biological processes, including proliferation, or synaptic transmission, is mediated and controlled by sophisticated protein machinery ensuring the preservation of the vital barrier function of the membrane throughout the process. Fusion of virus particles with host cell membranes is more sparingly arranged and often mediated by a single fusion protein, and the virus can afford to be less discriminative towards the possible different outcomes of fusion attempts. Formation of leaky intermediates was recently observed in some fusion processes, and an alternative trajectory of the process involving formation of π-shaped structures was suggested. In this study, we apply the methods of elasticity theory and Lagrangian formalism augmented by phenomenological and molecular geometry constraints and boundary conditions to investigate the traits of this trajectory and the drivers behind the choice of one of the possible scenarios depending on the properties of the system. The alternative pathway proved to be a dead end, and, depending on the parameters of the participating membranes and fusion proteins, the system can either reversibly enter the corresponding “leaky” configuration or be trapped in it. A parametric study in the biologically relevant range of variables emphasized the fusion protein properties crucial for the choice of the fusion scenario. View Full-Text
Keywords: membrane fusion; viral fusion; enveloped viruses; fusion peptides; stalk mechanism; dead-end state membrane fusion; viral fusion; enveloped viruses; fusion peptides; stalk mechanism; dead-end state
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Molotkovsky, R.J.; Galimzyanov, T.R.; Jiménez-Munguía, I.; Pavlov, K.V.; Batishchev, O.V.; Akimov, S.A. Switching between Successful and Dead-End Intermediates in Membrane Fusion. Int. J. Mol. Sci. 2017, 18, 2598.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top