Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes
Abstract
:1. Introduction
2. Results
2.1. Glycerol Release and Triacylglycerols (TAGs) Content in Undifferentiated 3T3-L1 Cells (UDCs), Untreated 3T3-L1 Cells Differentiated into Adipocytes (CTL-DCs) and Lipopolysaccharide-treated 3T3-L1 Cells Differentiated into Adipocytes (LPS-DCs)
2.2. Biophysical Analysis of UDCs, Untreated DCs and LPS-Treated DCs Membrane Permeability to Glycerol
2.3. AQP7, AQP9 and AQP3 Protein Expression in UDCs, CTL-DCs and LPS-DCs
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cells Culture
4.3. Glycerol Release Measurement
4.4. Triglyceride Measurement
4.5. Western Blot Analysis
4.6. Preparation of 3T3-L1 Plasma Membrane Vesicles
4.7. Stopped-Flow Light Scattering Measurement of Glycerol Permeability
4.8. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
AQP | Aquaporin |
CTL | Control |
DCs | 3T3-L1 cells differentiated into adipocytes |
LPS | Lipopolysaccharide |
UDCs | Undifferentiated 3T3-L1 cells |
TAGs | Triacylglycerols |
cAMP | Cyclic adenosine monophosphate |
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Chiadak, J.D.; Gena, P.; Gregoire, F.; Bolaky, N.; Delforge, V.; Perret, J.; Calamita, G.; Delporte, C. Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes. Int. J. Mol. Sci. 2017, 18, 2566. https://doi.org/10.3390/ijms18122566
Chiadak JD, Gena P, Gregoire F, Bolaky N, Delforge V, Perret J, Calamita G, Delporte C. Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes. International Journal of Molecular Sciences. 2017; 18(12):2566. https://doi.org/10.3390/ijms18122566
Chicago/Turabian StyleChiadak, Jeanne Durendale, Patrizia Gena, Françoise Gregoire, Nargis Bolaky, Valérie Delforge, Jason Perret, Giuseppe Calamita, and Christine Delporte. 2017. "Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes" International Journal of Molecular Sciences 18, no. 12: 2566. https://doi.org/10.3390/ijms18122566