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Int. J. Mol. Sci. 2017, 18(11), 2458; https://doi.org/10.3390/ijms18112458

Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain

1
National Institute of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea
2
Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
3
Division of Life Science, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 3 November 2017 / Revised: 15 November 2017 / Accepted: 15 November 2017 / Published: 18 November 2017
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to improve rice flour processing qualities through the transgenic expression of viscoelastic proteins in rice seeds. In this study, we characterized the transgenic expression of wheat glutenin subunits in rice seeds. The two genes 1Dx5_KK and 1Dy10_JK, which both encode wheat high-molecular-weight glutenin subunits that confer high dough elasticity, were cloned from Korean wheat cultivars KeumKang and JoKyung, respectively. These genes were inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter and were expressed in the high-amylose Korean rice cultivar Koami (Oryza sativa L.). Individual expression of both glutenin subunits was confirmed by SDS-PAGE and immunoblot analyses performed using T3 generation of transgenic rice seeds. The subcellular localization of 1Dx5_KK and 1Dy10_JK in the rice seed endosperm was confirmed by immunofluorescence analysis, indicating that the wheat glutenin subunits accumulate in protein body-II and novel protein body types in the rice seed. These results contribute to our understanding of engineered seed storage proteins in rice. View Full-Text
Keywords: seed storage proteins; high-molecular-weight glutenin subunits; 1Dx5_KK; 1Dy10_JK; glutelin; prolamin seed storage proteins; high-molecular-weight glutenin subunits; 1Dx5_KK; 1Dy10_JK; glutelin; prolamin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jo, Y.-M.; Cho, K.; Lee, H.-J.; Lim, S.-H.; Kim, J.S.; Kim, Y.-M.; Lee, J.-Y. Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain. Int. J. Mol. Sci. 2017, 18, 2458.

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