Coumestrol Epigenetically Suppresses Cancer Cell Proliferation: Coumestrol Is a Natural Haspin Kinase Inhibitor
Abstract
:1. Introduction
2. Results
2.1. Coumestrol Inhibits the Growth of Various Cancer Cell Types
2.2. Coumestrol Reduces Anchorage-Independent Cancer Cell Growth
2.3. Haspin Kinase Is a Direct Target of Coumestrol
2.4. Coumestrol Directly Interacts with Haspin Kinase
2.5. Haspin Kinase Modulates Cancer Cell Growth but Not Casein Kinase 2
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. Cell Proliferation Assay
4.4. Anchorage-Independent Cell Growth
4.5. Preparation of Coumestrol-Sepharose 4B Beads
4.6. Pull down Assay
4.7. Kinase Assay
4.8. Western Blot Analysis
4.9. Molecular Modeling
4.10. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kim, J.-E.; Lee, S.-Y.; Jang, M.; Choi, H.-K.; Kim, J.H.; Chen, H.; Lim, T.-G.; Dong, Z.; Lee, K.W. Coumestrol Epigenetically Suppresses Cancer Cell Proliferation: Coumestrol Is a Natural Haspin Kinase Inhibitor. Int. J. Mol. Sci. 2017, 18, 2228. https://doi.org/10.3390/ijms18102228
Kim J-E, Lee S-Y, Jang M, Choi H-K, Kim JH, Chen H, Lim T-G, Dong Z, Lee KW. Coumestrol Epigenetically Suppresses Cancer Cell Proliferation: Coumestrol Is a Natural Haspin Kinase Inhibitor. International Journal of Molecular Sciences. 2017; 18(10):2228. https://doi.org/10.3390/ijms18102228
Chicago/Turabian StyleKim, Jong-Eun, Sung-Young Lee, Mi Jang, Hyo-Kyung Choi, Jong Hun Kim, Hanyong Chen, Tae-Gyu Lim, Zigang Dong, and Ki Won Lee. 2017. "Coumestrol Epigenetically Suppresses Cancer Cell Proliferation: Coumestrol Is a Natural Haspin Kinase Inhibitor" International Journal of Molecular Sciences 18, no. 10: 2228. https://doi.org/10.3390/ijms18102228