α-Tocopherol at Nanomolar Concentration Protects Cortical Neurons against Oxidative Stress
AbstractThe aim of the present work is to study the mechanism of the α-tocopherol (α-T) protective action at nanomolar and micromolar concentrations against H2O2-induced brain cortical neuron death. The mechanism of α-T action on neurons at its nanomolar concentrations characteristic for brain extracellular space has not been practically studied yet. Preincubation with nanomolar and micromolar α-T for 18 h was found to increase the viability of cortical neurons exposed to H2O2; α-T effect was concentration-dependent in the nanomolar range. However, preincubation with nanomolar α-T for 30 min was not effective. Nanomolar and micromolar α-T decreased the reactive oxygen species accumulation induced in cortical neurons by the prooxidant. Using immunoblotting it was shown that preincubation with α-T at nanomolar and micromolar concentrations for 18 h prevented Akt inactivation and decreased PKCδ activation induced in cortical neurons by H2O2. α-T prevented the ERK1/2 sustained activation during 24 h caused by H2O2. α-T at nanomolar and micromolar concentrations prevented a great increase of the proapoptotic to antiapoptotic proteins (Bax/Bcl-2) ratio, elicited by neuron exposure to H2O2. The similar neuron protection mechanism by nanomolar and micromolar α-T suggests that a “more is better” approach to patients’ supplementation with vitamin E or α-T is not reasonable. View Full-Text
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Zakharova, I.O.; Sokolova, T.V.; Vlasova, Y.A.; Bayunova, L.V.; Rychkova, M.P.; Avrova, N.F. α-Tocopherol at Nanomolar Concentration Protects Cortical Neurons against Oxidative Stress. Int. J. Mol. Sci. 2017, 18, 216.
Zakharova IO, Sokolova TV, Vlasova YA, Bayunova LV, Rychkova MP, Avrova NF. α-Tocopherol at Nanomolar Concentration Protects Cortical Neurons against Oxidative Stress. International Journal of Molecular Sciences. 2017; 18(1):216.Chicago/Turabian Style
Zakharova, Irina O.; Sokolova, Tatiana V.; Vlasova, Yulia A.; Bayunova, Liubov V.; Rychkova, Maria P.; Avrova, Natalia F. 2017. "α-Tocopherol at Nanomolar Concentration Protects Cortical Neurons against Oxidative Stress." Int. J. Mol. Sci. 18, no. 1: 216.
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