Neurotrophin Promotes Neurite Outgrowth by Inhibiting Rif GTPase Activation Downstream of MAPKs and PI3K Signaling
Abstract
:1. Introduction
2. Results
2.1. Semaphorin 6A (Sema6A) Was as Effectively Neurotrophic as Nerve Growth Factor (NGF) in PC12 Cells
2.2. Mitogen-Activated Protein Kinase and Phosphoinositide 3-Kinase Signaling Were Necessary for Sema6A-Stimulated NGF-Induced Neurite Outgrowth in PC12 Cells
2.3. Rif Expression Antagonized Neurotrophin-Induced Neurite Outgrowth in PC12 Cells
2.4. Rif Activity Inhibited the Mitogenic Stimulation Mediated by Mitogen-Activated Protein Kinases (MAPKs) and Phosphatidylinositol-3-Kinase (PI3K) Activation
2.5. The Roles of Rif in PC12 Cell Neurite Formation
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Determination of Neurite Outgrowth
4.4. siRNA Transfection
4.5. Preparation of Recombinant Protein and Rif Activation Assay
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tian, X.; Yan, H.; Li, J.; Wu, S.; Wang, J.; Fan, L. Neurotrophin Promotes Neurite Outgrowth by Inhibiting Rif GTPase Activation Downstream of MAPKs and PI3K Signaling. Int. J. Mol. Sci. 2017, 18, 148. https://doi.org/10.3390/ijms18010148
Tian X, Yan H, Li J, Wu S, Wang J, Fan L. Neurotrophin Promotes Neurite Outgrowth by Inhibiting Rif GTPase Activation Downstream of MAPKs and PI3K Signaling. International Journal of Molecular Sciences. 2017; 18(1):148. https://doi.org/10.3390/ijms18010148
Chicago/Turabian StyleTian, Xiaoxia, Huijuan Yan, Jiayi Li, Shuang Wu, Junyu Wang, and Lifei Fan. 2017. "Neurotrophin Promotes Neurite Outgrowth by Inhibiting Rif GTPase Activation Downstream of MAPKs and PI3K Signaling" International Journal of Molecular Sciences 18, no. 1: 148. https://doi.org/10.3390/ijms18010148