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Int. J. Mol. Sci. 2016, 17(9), 1581; doi:10.3390/ijms17091581

Candida antarctica Lipase B Immobilized onto Chitin Conjugated with POSS® Compounds: Useful Tool for Rapeseed Oil Conversion

1
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, Poznan 60965, Poland
2
Institute of Chemical and Environment Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Pulaskiego 10, Szczecin 70322, Poland
3
Faculty of Chemistry, Adam Mickiewicz University in Poznan, Umultowska 89b, Poznan 61614, Poland
4
Poznan Science and Technology Park, Adam Mickiewicz University Foundation, Rubiez 46, Poznan 61612, Poland
5
Institute of Experimental Physics, Technische Universität Bergakademie Freiberg, Leipziger Str. 23, Freiberg 09599, Germany
*
Authors to whom correspondence should be addressed.
Academic Editor: Hitoshi Sashiwa
Received: 28 July 2016 / Revised: 8 September 2016 / Accepted: 9 September 2016 / Published: 20 September 2016
(This article belongs to the Special Issue Frontiers of Marine Biomaterials)
View Full-Text   |   Download PDF [2141 KB, uploaded 20 September 2016]   |  

Abstract

A new method is proposed for the production of a novel chitin-polyhedral oligomeric silsesquioxanes (POSS) enzyme support. Analysis by such techniques as X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy confirmed the effective functionalization of the chitin surface. The resulting hybrid carriers were used in the process of immobilization of the lipase type b from Candida antarctica (CALB). Fourier transform infrared spectroscopy (FTIR) confirmed the effective immobilization of the enzyme. The tests of the catalytic activity showed that the resulting support-biocatalyst systems remain hydrolytically active (retention of the hydrolytic activity up to 87% for the chitin + Methacryl POSS® cage mixture (MPOSS) + CALB after 24 h of the immobilization), as well as represents good thermal and operational stability, and retain over 80% of its activity in a wide range of temperatures (30–60 °C) and pH (6–9). Chitin-POSS-lipase systems were used in the transesterification processes of rapeseed oil at various reaction conditions. Produced systems allowed the total conversion of the oil to fatty acid methyl esters (FAME) and glycerol after 24 h of the process at pH 10 and a temperature 40 °C, while the Methacryl POSS® cage mixture (MPOSS) was used as a chitin-modifying agent. View Full-Text
Keywords: enzyme immobilization; chitin; polyhedral oligomeric silsesquioxanes; Candida antarctica lipase B; rapeseed oil transesterification enzyme immobilization; chitin; polyhedral oligomeric silsesquioxanes; Candida antarctica lipase B; rapeseed oil transesterification
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Zdarta, J.; Wysokowski, M.; Norman, M.; Kołodziejczak-Radzimska, A.; Moszyński, D.; Maciejewski, H.; Ehrlich, H.; Jesionowski, T. Candida antarctica Lipase B Immobilized onto Chitin Conjugated with POSS® Compounds: Useful Tool for Rapeseed Oil Conversion. Int. J. Mol. Sci. 2016, 17, 1581.

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