Next Article in Journal
Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit
Next Article in Special Issue
Cancer Immunology with a Focus on Understudied Cancers as Targets for Immunotherapy
Previous Article in Journal
Susceptibility and Immune Defence Mechanisms of Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) against Entomopathogenic Fungal Infections
Previous Article in Special Issue
Development of Novel Immunotherapies for Multiple Myeloma
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(9), 1525; doi:10.3390/ijms17091525

In Vitro Assessment of the Expression and T Cell Immunogenicity of the Tumor-Associated Antigens BORIS, MUC1, hTERT, MAGE-A3 and Sp17 in Uterine Cancer

Department of Oncology, Gynecologic Oncology, Campus Gasthuisberg, Sandra Tuyaerts, KU Leuven—University of Leuven, Herestraat 49 Box 818, B-3000 Leuven, Belgium
Department of Gynecology and Obstetrics, Division Gynecologic Oncology, University Hospitals Leuven, B-3000 Leuven, Belgium
Author to whom correspondence should be addressed.
Academic Editor: Michael R. Hamblin
Received: 11 July 2016 / Revised: 24 August 2016 / Accepted: 30 August 2016 / Published: 9 September 2016
View Full-Text   |   Download PDF [3107 KB, uploaded 9 September 2016]   |  


Background: While immunotherapy moved to the forefront of treatment of various cancers, it remains underexplored for uterine cancer. This might be due to the small patient population with advanced endometrial carcinoma and uterine sarcoma. Data about immunotherapeutic targets are scarce in endometrial carcinoma and lacking in uterine sarcoma. Methods: Expression of five tumor-associated antigens (TAA) (BORIS, MUC1, hTERT, MAGE-A3 and Sp17) was validated in uterine tumor samples by immunohistochemistry (IHC) and/or quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). TAA immunogenicity was analyzed by determining spontaneous T cell responses towards overlapping peptide pools covering the whole TAA in patient blood. Results: At mRNA level, MAGE-A3 and Sp17 were overexpressed in a minority of patients and BORIS was moderately overexpressed (26% in endometrial carcinoma and 62% in uterine sarcoma). hTERT was overexpressed in the vast majority of tumors. On protein level, MUC1 was upregulated in primary, recurrent and metastatic EMCAR and in metastatic US tumors. hTERT protein was highly expressed in both normal and malignant tissue. Spontaneous TAA-specific T cell responses were detected in a minority of patients, except for hTERT to which T cell responses occurred more frequently. Conclusions: These data point to MUC1 and hTERT as most suitable targets based on expression levels and T cell immunogenicity for use in immunotherapeutic regimens. View Full-Text
Keywords: tumor-associated antigens; expression; immunogenicity; endometrial carcinoma; uterine sarcoma tumor-associated antigens; expression; immunogenicity; endometrial carcinoma; uterine sarcoma

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vanderstraeten, A.; Tuyaerts, S.; Everaert, T.; Van Bree, R.; Verbist, G.; Luyten, C.; Amant, F. In Vitro Assessment of the Expression and T Cell Immunogenicity of the Tumor-Associated Antigens BORIS, MUC1, hTERT, MAGE-A3 and Sp17 in Uterine Cancer. Int. J. Mol. Sci. 2016, 17, 1525.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top