Next Article in Journal
Influence of Preoperative Serum Aspartate Aminotransferase (AST) Level on the Prognosis of Patients with Non-Small Cell Lung Cancer
Next Article in Special Issue
Effects of the Activin A–Follistatin System on Myocardial Cell Apoptosis through the Endoplasmic Reticulum Stress Pathway in Heart Failure
Previous Article in Journal
Phyllanthus emblica Fruit Extract Activates Spindle Assembly Checkpoint, Prevents Mitotic Aberrations and Genomic Instability in Human Colon Epithelial NCM460 Cells
Previous Article in Special Issue
Targeting the Innate Immune Response to Improve Cardiac Graft Recovery after Heart Transplantation: Implications for the Donation after Cardiac Death
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(9), 1471; doi:10.3390/ijms17091471

T-Tubular Electrical Defects Contribute to Blunted β-Adrenergic Response in Heart Failure

1
European Laboratory for Non-Linear Spectroscopy, Florence 50019, Italy
2
National Institute of Optics, National Research Council, Florence 50125, Italy
3
Division of Pharmacology, Department “NeuroFarBa”, University of Florence, Florence 50139, Italy
4
Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy
5
R. D. Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
6
Department of Physics and Astronomy, University of Florence, Sesto Fiorentino 50019, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: H. W. M. Niessen and Paul A. J. Krijnen
Received: 12 July 2016 / Revised: 17 August 2016 / Accepted: 30 August 2016 / Published: 3 September 2016
(This article belongs to the Special Issue Improvement of Cardiac Function in Heart Failure 2017)
View Full-Text   |   Download PDF [1073 KB, uploaded 3 September 2016]   |  

Abstract

Alterations of the β-adrenergic signalling, structural remodelling, and electrical failure of T-tubules are hallmarks of heart failure (HF). Here, we assess the effect of β-adrenoceptor activation on local Ca2+ release in electrically coupled and uncoupled T-tubules in ventricular myocytes from HF rats. We employ an ultrafast random access multi-photon (RAMP) microscope to simultaneously record action potentials and Ca2+ transients from multiple T-tubules in ventricular cardiomyocytes from a HF rat model of coronary ligation compared to sham-operated rats as a control. We confirmed that β-adrenergic stimulation increases the frequency of Ca2+ sparks, reduces Ca2+ transient variability, and hastens the decay of Ca2+ transients: all these effects are similarly exerted by β-adrenergic stimulation in control and HF cardiomyocytes. Conversely, β-adrenergic stimulation in HF cells accelerates a Ca2+ rise exclusively in the proximity of T-tubules that regularly conduct the action potential. The delayed Ca2+ rise found at T-tubules that fail to conduct the action potential is instead not affected by β-adrenergic signalling. Taken together, these findings indicate that HF cells globally respond to β-adrenergic stimulation, except at T-tubules that fail to conduct action potentials, where the blunted effect of the β-adrenergic signalling may be directly caused by the lack of electrical activity. View Full-Text
Keywords: heart failure; T-tubules; excitation-contraction coupling; β-adrenergic signalling; non-linear microscopy imaging heart failure; T-tubules; excitation-contraction coupling; β-adrenergic signalling; non-linear microscopy imaging
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Crocini, C.; Coppini, R.; Ferrantini, C.; Yan, P.; Loew, L.M.; Poggesi, C.; Cerbai, E.; Pavone, F.S.; Sacconi, L. T-Tubular Electrical Defects Contribute to Blunted β-Adrenergic Response in Heart Failure. Int. J. Mol. Sci. 2016, 17, 1471.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top