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Int. J. Mol. Sci. 2016, 17(8), 1251; doi:10.3390/ijms17081251

Drought-Induced Leaf Proteome Changes in Switchgrass Seedlings

1
Department of Agricultural Sciences, Tennessee State University, 3500 John Merritt Blvd, Nashville, TN 37209, USA
2
Department of Biological Sciences, Tennessee State University, 3500 John Merritt Blvd, Nashville, TN 37209, USA
3
Functional & Comparative Proteomics Center, USDA-ARS, Cornell University, Ithaca, NY 14853, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Setsuko Komatsu
Received: 21 June 2016 / Revised: 24 July 2016 / Accepted: 27 July 2016 / Published: 2 August 2016
(This article belongs to the Special Issue Plant Proteomic Research)
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Abstract

Switchgrass (Panicum virgatum) is a perennial crop producing deep roots and thus highly tolerant to soil water deficit conditions. However, seedling establishment in the field is very susceptible to prolonged and periodic drought stress. In this study, a “sandwich” system simulating a gradual water deletion process was developed. Switchgrass seedlings were subjected to a 20-day gradual drought treatment process when soil water tension was increased to 0.05 MPa (moderate drought stress) and leaf physiological properties had expressed significant alteration. Drought-induced changes in leaf proteomes were identified using the isobaric tags for relative and absolute quantitation (iTRAQ) labeling method followed by nano-scale liquid chromatography mass spectrometry (nano-LC-MS/MS) analysis. Additionally, total leaf proteins were processed using a combinatorial library of peptide ligands to enrich for lower abundance proteins. Both total proteins and those enriched samples were analyzed to increase the coverage of the quantitative proteomics analysis. A total of 7006 leaf proteins were identified, and 257 (4% of the leaf proteome) expressed a significant difference (p < 0.05, fold change <0.6 or >1.7) from the non-treated control to drought-treated conditions. These proteins are involved in the regulation of transcription and translation, cell division, cell wall modification, phyto-hormone metabolism and signaling transduction pathways, and metabolic pathways of carbohydrates, amino acids, and fatty acids. A scheme of abscisic acid (ABA)-biosynthesis and ABA responsive signal transduction pathway was reconstructed using these drought-induced significant proteins, showing systemic regulation at protein level to deploy the respective mechanism. Results from this study, in addition to revealing molecular responses to drought stress, provide a large number of proteins (candidate genes) that can be employed to improve switchgrass seedling growth and establishment under soil drought conditions (Data are available via ProteomeXchange with identifier PXD004675). View Full-Text
Keywords: physiological properties; isobaric tags for relative and absolute quantitation (iTRAQ); ProteoMiner; functional pathways; abscisic acid (ABA) signaling; “Sandwich” plant growth system physiological properties; isobaric tags for relative and absolute quantitation (iTRAQ); ProteoMiner; functional pathways; abscisic acid (ABA) signaling; “Sandwich” plant growth system
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ye, Z.; Sangireddy, S.; Okekeogbu, I.; Zhou, S.; Yu, C.-L.; Hui, D.; Howe, K.J.; Fish, T.; Thannhauser, T.W. Drought-Induced Leaf Proteome Changes in Switchgrass Seedlings. Int. J. Mol. Sci. 2016, 17, 1251.

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