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Int. J. Mol. Sci. 2016, 17(7), 1154; doi:10.3390/ijms17071154

The OsCYP19-4 Gene Is Expressed as Multiple Alternatively Spliced Transcripts Encoding Isoforms with Distinct Cellular Localizations and PPIase Activities under Cold Stress

1
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon 34113, Korea
2
Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
3
Department of Pharmacology, College of Medicine, Seonam University, Namwon 55724, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Akila Mayeda
Received: 5 May 2016 / Revised: 5 July 2016 / Accepted: 8 July 2016 / Published: 19 July 2016
(This article belongs to the Special Issue Pre-mRNA Splicing 2016)
View Full-Text   |   Download PDF [2444 KB, uploaded 19 July 2016]   |  

Abstract

Alternative splicing (AS) is an important molecular mechanism by which single genes can generate multiple mRNA isoforms. We reported previously that, in Oryza sativa, the cyclophilin 19-4 (OsCYP19-4.1) transcript was significantly upregulated in response to cold stress, and that transgenic plants were cold tolerant. Here we show that, under cold stress, OsCYP19-4 produces eight transcript variants by intron retention and exon skipping, resulting in production of four distinct protein isoforms. The OsCYP19-4 AS isoforms exhibited different cellular localizations in the epidermal cells: in contrast to OsCYP19-4.1, the OsCYP19-4.2 and OsCYP19-4.3 proteins were primarily targeted to guard and subsidiary cells, whereas OsCYP19-4.5, which consists largely of an endoplasmic reticulum (ER) targeting signal, was co-localized with the RFP-BiP marker in the ER. In OsCYP19-4.2, the key residues of the PPIase domain are altered; consistent with this, recombinant OsCYP19-4.2 had significantly lower PPIase activity than OsCYP19-4.1 in vitro. Specific protein-protein interactions between OsCYP19-4.2/3 and AtRCN1 were verified in yeast two-hybrid (Y2H) and bimolecular fluoresence complementation (BiFC assays), although the OsCYP19-4 isoforms could not bind each other. Based on these results, we propose that two OsCYP19-4 AS isoforms, OsCYP19-4.2 and OsCYP19-4.3, play roles linking auxin transport and cold stress via interactions with RCN1. View Full-Text
Keywords: alternative splicing; cold stress; isoform; OsCYP19-4; PPIase activity; RCN1 interactor alternative splicing; cold stress; isoform; OsCYP19-4; PPIase activity; RCN1 interactor
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MDPI and ACS Style

Lee, A.; Lee, S.S.; Jung, W.Y.; Park, H.J.; Lim, B.R.; Kim, H.-S.; Ahn, J.C.; Cho, H.S. The OsCYP19-4 Gene Is Expressed as Multiple Alternatively Spliced Transcripts Encoding Isoforms with Distinct Cellular Localizations and PPIase Activities under Cold Stress. Int. J. Mol. Sci. 2016, 17, 1154.

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