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Int. J. Mol. Sci. 2016, 17(7), 1086; doi:10.3390/ijms17071086

Dual-Labeled Near-Infrared/99mTc Imaging Probes Using PAMAM-Coated Silica Nanoparticles for the Imaging of HER2-Expressing Cancer Cells

1
Quantitative Diagnostic Imaging Program, Graduate School of Life Dentistry at Niigata, The Nippon Dental University, Niigata 951-8580, Japan
2
Department of Oral and Maxillofacial Radiology, The Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan
3
Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan
4
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
5
Faculty of Engineering, Niigata University, Niigata 950-2181, Japan
Current address: Haruka Yamaguchi-Takezawa, Department of Life Science Dentistry, The Nippon Dental University, Niigata 951-8580, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Vladimir Sivakov
Received: 24 May 2016 / Revised: 1 July 2016 / Accepted: 4 July 2016 / Published: 7 July 2016
(This article belongs to the Special Issue Inorganic Nanostructures in Biological Systems)
View Full-Text   |   Download PDF [11954 KB, uploaded 7 July 2016]   |  

Abstract

We sought to develop dual-modality imaging probes using functionalized silica nanoparticles to target human epidermal growth factor receptor 2 (HER2)-overexpressing breast cancer cells and achieve efficient target imaging of HER2-expressing tumors. Polyamidoamine-based functionalized silica nanoparticles (PCSNs) for multimodal imaging were synthesized with near-infrared (NIR) fluorescence (indocyanine green (ICG)) and technetium-99m (99mTc) radioactivity. Anti-HER2 antibodies were bound to the labeled PCSNs. These dual-imaging probes were tested to image HER2-overexpressing breast carcinoma cells. In vivo imaging was also examined in breast tumor xenograft models in mice. SK-BR3 (HER2 positive) cells were imaged with stronger NIR fluorescent signals than that in MDA-MB231 (HER2 negative) cells. The increased radioactivity of the SK-BR3 cells was also confirmed by phosphor imaging. NIR images showed strong fluorescent signals in the SK-BR3 tumor model compared to muscle tissues and the MDA-MB231 tumor model. Automatic well counting results showed increased radioactivity in the SK-BR3 xenograft tumors. We developed functionalized silica nanoparticles loaded with 99mTc and ICG for the targeting and imaging of HER2-expressing cells. The dual-imaging probes efficiently imaged HER2-overexpressing cells. Although further studies are needed to produce efficient isotope labeling, the results suggest that the multifunctional silica nanoparticles are a promising vehicle for imaging specific components of the cell membrane in a dual-modality manner. View Full-Text
Keywords: dual-imaging probe; silica nanoparticles; HER2; 99mTc; NIR; ICG; SK-BR3; MDA-MB231; athymic mice; xenograft dual-imaging probe; silica nanoparticles; HER2; 99mTc; NIR; ICG; SK-BR3; MDA-MB231; athymic mice; xenograft
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yamaguchi, H.; Tsuchimochi, M.; Hayama, K.; Kawase, T.; Tsubokawa, N. Dual-Labeled Near-Infrared/99mTc Imaging Probes Using PAMAM-Coated Silica Nanoparticles for the Imaging of HER2-Expressing Cancer Cells. Int. J. Mol. Sci. 2016, 17, 1086.

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