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Int. J. Mol. Sci. 2016, 17(7), 1058; doi:10.3390/ijms17071058

Transcriptome Sequencing Analyses between the Cytoplasmic Male Sterile Line and Its Maintainer Line in Welsh Onion (Allium fistulosum L.)

1
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing 100097, China
2
Institute of Agricultural Integrated Development, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 1 April 2016 / Revised: 24 June 2016 / Accepted: 27 June 2016 / Published: 1 July 2016
(This article belongs to the Section Molecular Botany)
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Abstract

Cytoplasmic male sterility (CMS) is important for exploiting heterosis in crop plants and also serves as a model for investigating nuclear–cytoplasmic interaction. The molecular mechanism of cytoplasmic male sterility and fertility restoration was investigated in several important economic crops but remains poorly understood in the Welsh onion. Therefore, we compared the differences between the CMS line 64-2 and its maintainer line 64-1 using transcriptome sequencing with the aim of determining critical genes and pathways associated with male sterility. This study combined two years of RNA-seq data; there were 1504 unigenes (in May 2013) and 2928 unigenes (in May 2014) that were differentially expressed between the CMS and cytoplasmic male maintainer Welsh onion varieties. Known CMS-related genes were found in the set of differentially expressed genes and checked by qPCR. These genes included F-type ATPase, NADH dehydrogenase, cytochrome c oxidase, etc. Overall, this study demonstrated that the CMS regulatory genes and pathways may be associated with the mitochondria and nucleus in the Welsh onion. We believe that this transcriptome dataset will accelerate the research on CMS gene clones and other functional genomics research on A. fistulosum L. View Full-Text
Keywords: Welsh onion; transcriptome sequencing; cytoplasmic male sterility (CMS) Welsh onion; transcriptome sequencing; cytoplasmic male sterility (CMS)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, Q.; Lan, Y.; Wen, C.; Zhao, H.; Wang, J.; Wang, Y. Transcriptome Sequencing Analyses between the Cytoplasmic Male Sterile Line and Its Maintainer Line in Welsh Onion (Allium fistulosum L.). Int. J. Mol. Sci. 2016, 17, 1058.

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