Next Article in Journal / Special Issue
All-Trans Retinoic Acid Modulates DNA Damage Response and the Expression of the VEGF-A and MKI67 Genes in ARPE-19 Cells Subjected to Oxidative Stress
Previous Article in Journal
Toxicological Considerations, Toxicity Assessment, and Risk Management of Inhaled Nanoparticles
Previous Article in Special Issue
Ectopic Expression of Testis Germ Cell Proteins in Cancer and Its Potential Role in Genomic Instability
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(6), 931; doi:10.3390/ijms17060931

Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response

1
Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 300, Taiwan
2
Department of Bioscience Technology, Chung Yuan Christian University, Chungli 320, Taiwan
3
Institute of Preventive Medicine, National Defense Medical Center, Taipei 237, Taiwan
4
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Guillermo T. Sáez
Received: 14 March 2016 / Revised: 21 May 2016 / Accepted: 7 June 2016 / Published: 14 June 2016
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases)
View Full-Text   |   Download PDF [5188 KB, uploaded 15 June 2016]   |  

Abstract

BacMam is an insect-derived recombinant baculovirus that can deliver genes into mammalian cells. BacMam vectors carrying target genes are able to enter a variety of cell lines by endocytosis, but the level of expression of the transgene depends on the cell line and the state of the transduced cells. In this study, we demonstrated that the DNA damage response (DDR) could act as an alternative pathway to boost the transgene(s) expression by BacMam and be comparable to the inhibitors of histone deacetylase. Topoisomerase II (Top II) inhibitor-induced DDR can enhance the CMV-IE/enhancer mediated gene expression up to 12-fold in BacMam-transduced U-2OS cells. The combination of a Top II inhibitor, VM-26, can also augment the killing efficiency of a p53-expressing BacMam vector in U-2OS osteosarcoma cells. These results open a new avenue to facilitate the application of BacMam for gene delivery and therapy. View Full-Text
Keywords: baculovirus; BacMam; DNA damage response; topoisomerase; p53 baculovirus; BacMam; DNA damage response; topoisomerase; p53
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, M.-K.; Lin, J.-J.; Chen, C.-Y.; Kuo, S.-C.; Wang, Y.-M.; Chan, H.-L.; Wu, T.Y. Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response. Int. J. Mol. Sci. 2016, 17, 931.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top