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Int. J. Mol. Sci. 2016, 17(6), 910; doi:10.3390/ijms17060910

The CLC-2 Chloride Channel Modulates ECM Synthesis, Differentiation, and Migration of Human Conjunctival Fibroblasts via the PI3K/Akt Signaling Pathway

1
Department of Ophthalmology, Second Hospital of Jilin University, Jilin University, Changchun 130041, China
2
Department of Ophthalmology, China-Japan Union Hospital, Jilin University, Changchun 130031, China
*
Author to whom correspondence should be addressed.
Academic Editor: Atsushi Matsuzawa
Received: 6 May 2016 / Revised: 27 May 2016 / Accepted: 30 May 2016 / Published: 9 June 2016
(This article belongs to the Special Issue Kinase Signal Transduction)
View Full-Text   |   Download PDF [5461 KB, uploaded 9 June 2016]   |  

Abstract

Recent evidence suggests that chloride channels are critical for cell proliferation, migration, and differentiation. We examined the effects of transforming growth factor (TGF)-β1 on chloride channel expression and associations with human conjunctival fibroblast (HConF) biology. To investigate the potential role of chloride channel (CLC)-2 in migration, transition to myofibroblasts and extracellular matrix (ECM) synthesis of HconF, a small interfering RNA (siRNA) approach was applied. TGF-β1-induced migration and transition of fibroblasts to myofibroblasts characterized by α-smooth muscle actin (α-SMA) expression, supported by increased endogenous expression of CLC-2 protein and mRNA transcripts. ECM (collagen I and fibronectin) synthesis in HConF was enhanced by TGF-β1. CLC-2 siRNA treatment reduced TGF-β1-induced cell migration, transition of fibroblasts to myofibroblasts, and ECM synthesis of HConF. CLC-2 siRNA treatment in the presence of TGF-β1 inhibited phosphorylation of PI3K and Akt in HConF. These findings demonstrate that CLC-2 chloride channels are important for TGF-β1-induced migration, differentiation, and ECM synthesis via PI3K/Akt signaling in HConF. View Full-Text
Keywords: CLC-2 siRNA; TGF-β1 differentiation; extracellular matrix; human conjunctival fibroblasts; migration; signaling pathway CLC-2 siRNA; TGF-β1 differentiation; extracellular matrix; human conjunctival fibroblasts; migration; signaling pathway
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Sun, L.; Dong, Y.; Zhao, J.; Yin, Y.; Zheng, Y. The CLC-2 Chloride Channel Modulates ECM Synthesis, Differentiation, and Migration of Human Conjunctival Fibroblasts via the PI3K/Akt Signaling Pathway. Int. J. Mol. Sci. 2016, 17, 910.

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