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Int. J. Mol. Sci. 2016, 17(6), 902; doi:10.3390/ijms17060902

High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

1
Laboratorio de Biotecnología 1, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito 1, Nuevo Campus Universitario, Chihuahua CP 31125, Mexico
2
Laboratorio de Biotecnología, Campus de Ciencias Agropecuarias, Universidad Autónoma de Nuevo León, Francisco Villa S/N Col. Ex hacienda El Canadá, General Escobedo, Nuevo León 66054, Mexico
*
Author to whom correspondence should be addressed.
Academic Editors: Ana Maria Gómez Caravaca and Antonio Segura-Carretero
Received: 29 April 2016 / Revised: 1 June 2016 / Accepted: 1 June 2016 / Published: 9 June 2016
(This article belongs to the Special Issue Advances in Molecular Research of Functional and Nutraceutical Food)
View Full-Text   |   Download PDF [1610 KB, uploaded 9 June 2016]   |  

Abstract

In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin) demonstrated antibacterial activity against Escherichia coli (E. coli) BL21DE3, Staphylococcus aureus (S. aureus) FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa) ATCC 27833. The successful expression and characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly. View Full-Text
Keywords: recombinant bovine lactoferrin; lactoferricin; antibacterial activity; Pichia pastoris recombinant bovine lactoferrin; lactoferricin; antibacterial activity; Pichia pastoris
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MDPI and ACS Style

Iglesias-Figueroa, B.; Valdiviezo-Godina, N.; Siqueiros-Cendón, T.; Sinagawa-García, S.; Arévalo-Gallegos, S.; Rascón-Cruz, Q. High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity. Int. J. Mol. Sci. 2016, 17, 902.

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