Next Article in Journal
Human Saliva Collection Devices for Proteomics: An Update
Next Article in Special Issue
Molecular Signals Controlling the Inhibition of Nodulation by Nitrate in Medicago truncatula
Previous Article in Journal
A Nonlinear Model for Gene-Based Gene-Environment Interaction
Previous Article in Special Issue
Metabolomic Profiling of Bradyrhizobium diazoefficiens-Induced Root Nodules Reveals Both Host Plant-Specific and Developmental Signatures
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(6), 887; doi:10.3390/ijms17060887

Regulation of Small RNAs and Corresponding Targets in Nod Factor-Induced Phaseolus vulgaris Root Hair Cells

1
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 1001, Cuernavaca 62210, Morelos, Mexico
2
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología (UNAM), Avenida Universidad 2001, Cuernavaca 62210, Morelos, Mexico
*
Author to whom correspondence should be addressed.
Academic Editors: Peter M. Gresshoff and Brett Ferguson
Received: 29 April 2016 / Revised: 26 May 2016 / Accepted: 27 May 2016 / Published: 4 June 2016
(This article belongs to the Special Issue Molecular Signals in Nodulation Control)
View Full-Text   |   Download PDF [1497 KB, uploaded 4 June 2016]   |  

Abstract

A genome-wide analysis identified the set of small RNAs (sRNAs) from the agronomical important legume Phaseolus vulgaris (common bean), including novel P. vulgaris-specific microRNAs (miRNAs) potentially important for the regulation of the rhizobia-symbiotic process. Generally, novel miRNAs are difficult to identify and study because they are very lowly expressed in a tissue- or cell-specific manner. In this work, we aimed to analyze sRNAs from common bean root hairs (RH), a single-cell model, induced with pure Rhizobium etli nodulation factors (NF), a unique type of signal molecule. The sequence analysis of samples from NF-induced and control libraries led to the identity of 132 mature miRNAs, including 63 novel miRNAs and 1984 phasiRNAs. From these, six miRNAs were significantly differentially expressed during NF induction, including one novel miRNA: miR-RH82. A parallel degradome analysis of the same samples revealed 29 targets potentially cleaved by novel miRNAs specifically in NF-induced RH samples; however, these novel miRNAs were not differentially accumulated in this tissue. This study reveals Phaseolus vulgaris-specific novel miRNA candidates and their corresponding targets that meet all criteria to be involved in the regulation of the early nodulation events, thus setting the basis for exploring miRNA-mediated improvement of the common bean–rhizobia symbiosis. View Full-Text
Keywords: common bean; legume-rhizobia interaction; miRNAs; phasiRNAs common bean; legume-rhizobia interaction; miRNAs; phasiRNAs
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Formey, D.; Martín-Rodríguez, J.Á.; Leija, A.; Santana, O.; Quinto, C.; Cárdenas, L.; Hernández, G. Regulation of Small RNAs and Corresponding Targets in Nod Factor-Induced Phaseolus vulgaris Root Hair Cells. Int. J. Mol. Sci. 2016, 17, 887.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top