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Int. J. Mol. Sci. 2016, 17(6), 883; doi:10.3390/ijms17060883

Transcriptional Profiling and miRNA-Target Network Analysis Identify Potential Biomarkers for Efficacy Evaluation of Fuzheng-Huayu Formula-Treated Hepatitis B Caused Liver Cirrhosis

1
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2
Laboratory of Epigenetics, Institute of Biomedical Science, Fudan University, Shanghai 200032, China
3
Department of Endocrinology and Metabolism, Shanghai First People’s Hospital, Shanghai Jiaotong University, Shanghai 201201, China
4
Institute of Liver Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
5
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 21 April 2016 / Revised: 26 May 2016 / Accepted: 27 May 2016 / Published: 3 June 2016
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [3154 KB, uploaded 3 June 2016]   |  

Abstract

Fuzheng-Huayu (FZHY) formula has been found to have a satisfactory effect on hepatitis B-caused cirrhosis (HBC) treatment. However, the efficacy evaluation of FZHY is often challenging. In this study, a randomized, double-blind and placebo-controlled trial was used to evaluate the therapeutic efficacy of FZHY in HBC treatment. In the trial, 35 medical indexes were detected, and 14 indexes had a statistically-significant difference before compared to after the trial. Importantly, the Child-Pugh score also demonstrated FZHY having therapeutic efficacy. Furthermore, the microRNA (miRNA) profiles of 12 serum samples were detected in FZHY groups, and 112 differential-expressed (DE) miRNAs were determined. Using predicted miRNA targets, 13 kernel miRNAs were identified from the established miRNA-target network. Subsequently, quantitative Real-time Polymerase Chain Reaction (qRT-PCR) was used to validate the expression level of 13 identified miRNAs in the trials. The results showed that nine miRNAs have a statistically-significant difference before compared to after FZHY treatment. By means of a logistic regression model, a miRNA panel with hsa-miR-18a-5p, -326, -1182 and -193b-5p was established, and it can clearly improve the accuracy of the efficacy evaluation of FZHY. This study suggested that the particular miRNAs can act as potential biomarkers and obviously increase the diagnostic accuracy for drug evaluation in HBC treatment progression. View Full-Text
Keywords: Fuzheng-Huayu (FZHY) formula; hepatitis B-caused cirrhosis (HBC); microRNA; miRNA-target network Fuzheng-Huayu (FZHY) formula; hepatitis B-caused cirrhosis (HBC); microRNA; miRNA-target network
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, Q.; Wu, F.; Wang, M.; Dong, S.; Liu, Y.; Lu, Y.; Song, Y.; Zhou, Q.; Liu, P.; Luo, Y.; Su, S. Transcriptional Profiling and miRNA-Target Network Analysis Identify Potential Biomarkers for Efficacy Evaluation of Fuzheng-Huayu Formula-Treated Hepatitis B Caused Liver Cirrhosis. Int. J. Mol. Sci. 2016, 17, 883.

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