Next Article in Journal
Host Plants Identification for Adult Agrotis ipsilon, a Long-Distance Migratory Insect
Next Article in Special Issue
Melanoma-Derived BRAFV600E Mutation in Peritumoral Stromal Cells: Implications for in Vivo Cell Fusion
Previous Article in Journal
Review on Graph Clustering and Subgraph Similarity Based Analysis of Neurological Disorders
Previous Article in Special Issue
Dendritic-Tumor Fusion Cell-Based Cancer Vaccines
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(6), 826; doi:10.3390/ijms17060826

Melanoma Cells Can Adopt the Phenotype of Stromal Fibroblasts and Macrophages by Spontaneous Cell Fusion in Vitro

1
Department of Dermatology and Allergology, University of Szeged, Szeged 6720, Hungary
2
MTA-SZTE Dermatological Research Group, Szeged 6720, Hungary
3
Institute of Immunology & Experimental Oncology, Witten/Herdecke University, Witten 58453, Germany
Lajos V. Kemény and Zsuzsanna Kurgyis contributed equally to this work.
Lajos Kemény and István B. Németh contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Anthony Lemarié
Received: 31 March 2016 / Revised: 10 May 2016 / Accepted: 17 May 2016 / Published: 2 June 2016
(This article belongs to the Special Issue Cell Fusion in Cancer)
View Full-Text   |   Download PDF [4763 KB, uploaded 2 June 2016]   |  

Abstract

After the removal of primary cutaneous melanoma some patients develop local recurrences, even after having histologically tumor-free re-excision. A potential explanation behind this phenomenon is that tumor cells switch their phenotype, making their recognition via standard histopathological assessments extremely difficult. Tumor-stromal cell fusion has been proposed as a potential mechanism for tumor cells to acquire mesenchymal traits; therefore, we hypothesized that melanoma cells could acquire fibroblast- and macrophage-like phenotypes via cell fusion. We show that melanoma cells spontaneously fuse with human dermal fibroblasts and human peripheral blood monocytes in vitro. The hybrid cells’ nuclei contain chromosomes from both parental cells and are indistinguishable from the parental fibroblasts or macrophages based on their morphology and immunophenotype, as they could lose the melanoma specific MART1 marker, but express the fibroblast marker smooth muscle actin or the macrophage marker CD68. Our results suggest that, by spontaneous cell fusion in vitro, tumor cells can adopt the morphology and immunophenotype of stromal cells while still carrying oncogenic, tumor-derived genetic information. Therefore, melanoma–stromal cell fusion might play a role in missing tumor cells by routine histopathological assessments. View Full-Text
Keywords: cell fusion; spontaneous melanoma; macrophage; fibroblast cell fusion; spontaneous melanoma; macrophage; fibroblast
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kemény, L.V.; Kurgyis, Z.; Buknicz, T.; Groma, G.; Jakab, Á.; Zänker, K.; Dittmar, T.; Kemény, L.; Németh, I.B. Melanoma Cells Can Adopt the Phenotype of Stromal Fibroblasts and Macrophages by Spontaneous Cell Fusion in Vitro. Int. J. Mol. Sci. 2016, 17, 826.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top