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Int. J. Mol. Sci. 2016, 17(6), 851; doi:10.3390/ijms17060851

Host Plants Identification for Adult Agrotis ipsilon, a Long-Distance Migratory Insect

1
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, China
*
Author to whom correspondence should be addressed.
Academic Editor: Massimo Maffei
Received: 24 April 2016 / Revised: 21 May 2016 / Accepted: 23 May 2016 / Published: 2 June 2016
(This article belongs to the Special Issue Plant-Insect Interactions)
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Abstract

In this study, we determined the host relationship of Agrotis ipsilon moths by identifying pollen species adhering them during their long-distance migration. Pollen carried by A. ipsilon moths was collected from 2012 to 2014 on a small island in the center of the Bohai Strait, which is a seasonal migration pathway of this pest species. Genomic DNA of single pollen grains was amplified by using whole genome amplification technology, and a portion of the chloroplast rbcL sequence was then amplified from this material. Pollen species were identified by a combination of DNA barcoding and pollen morphology. We found 28 species of pollen from 18 families on the tested moths, mainly from Angiosperm, Dicotyledoneae. From this, we were able to determine that these moths visit woody plants more than herbaceous plants that they carry more pollen in the early and late stages of the migration season, and that the amounts of pollen transportation were related to moth sex, moth body part, and plant species. In general, 31% of female and 26% of male moths were found to be carrying pollen. Amounts of pollen on the proboscis was higher for female than male moths, while the reverse was true for pollen loads on the antennae. This work provides a new approach to study the interactions between noctuid moth and their host plants. Identification of plant hosts for adult moths furthers understanding of the coevolution processes between moths and their host plants. View Full-Text
Keywords: Agrotis ipsilon; pollen; molecular identification; “insect-host plant” coevolution Agrotis ipsilon; pollen; molecular identification; “insect-host plant” coevolution
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, Y.; Fu, X.; Mao, L.; Xing, Z.; Wu, K. Host Plants Identification for Adult Agrotis ipsilon, a Long-Distance Migratory Insect. Int. J. Mol. Sci. 2016, 17, 851.

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