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Int. J. Mol. Sci. 2016, 17(6), 783; doi:10.3390/ijms17060783

Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control

1
College of Medicine, Foshan University, Foshan 528000, China
2
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
3
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China
4
School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China
*
Author to whom correspondence should be addressed.
Academic Editors: Jun Li and Li Lin
Received: 14 April 2016 / Revised: 9 May 2016 / Accepted: 16 May 2016 / Published: 30 May 2016
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [1761 KB, uploaded 30 May 2016]   |  

Abstract

In mammals, leptin is a peripheral satiety factor that inhibits feeding by regulating a variety of appetite-related hormones in the brain. However, most of the previous studies examining leptin in fish feeding were performed with mammalian leptins, which share very low sequence homologies with fish leptins. To elucidate the function and mechanism of endogenous fish leptins in feeding regulation, recombinant goldfish leptin-AI and leptin-AII were expressed in methylotrophic yeast and purified by immobilized metal ion affinity chromatography (IMAC). By intraperitoneal (IP) injection, both leptin-AI and leptin-AII were shown to inhibit the feeding behavior and to reduce the food consumption of goldfish in 2 h. In addition, co-treatment of leptin-AI or leptin-AII could block the feeding behavior and reduce the food consumption induced by neuropeptide Y (NPY) injection. High levels of leptin receptor (lepR) mRNA were detected in the hypothalamus, telencephalon, optic tectum and cerebellum of the goldfish brain. The appetite inhibitory effects of leptins were mediated by downregulating the mRNA levels of orexigenic NPY, agouti-related peptide (AgRP) and orexin and upregulating the mRNA levels of anorexigenic cocaine-amphetamine-regulated transcript (CART), cholecystokinin (CCK), melanin-concentrating hormone (MCH) and proopiomelanocortin (POMC) in different areas of the goldfish brain. Our study, as a whole, provides new insights into the functions and mechanisms of leptins in appetite control in a fish model. View Full-Text
Keywords: leptin; goldfish; methylotrophic yeast; feeding control; gene regulation; appetite regulators leptin; goldfish; methylotrophic yeast; feeding control; gene regulation; appetite regulators
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Yan, A.-F.; Chen, T.; Chen, S.; Ren, C.-H.; Hu, C.-Q.; Cai, Y.-M.; Liu, F.; Tang, D.-S. Goldfish Leptin-AI and Leptin-AII: Function and Central Mechanism in Feeding Control. Int. J. Mol. Sci. 2016, 17, 783.

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