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Int. J. Mol. Sci. 2016, 17(5), 756; doi:10.3390/ijms17050756

Conditional Knockout in Mice Reveals the Critical Roles of Ppp2ca in Epidermis Development

1
,
2
and
1,3,4,5,6,*
1
Department of Pharmacology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China
2
Translational Medicine Center, Yancheng No. 1 People’s Hospital, Yancheng 224000, China
3
Model Animal Research Center of Nanjing Medical University, Nanjing 210029, China
4
Key Laboratory of National Reproductive Medicine Department of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing 210029, China
5
Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University Nanjing, Nanjing 210029, China
6
Department of cell biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China
*
Author to whom correspondence should be addressed.
Academic Editor: Terrence Piva
Received: 23 March 2016 / Revised: 9 May 2016 / Accepted: 11 May 2016 / Published: 18 May 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [14758 KB, uploaded 18 May 2016]   |  

Abstract

The epidermis is an important tissue in Homo sapines and other animals, and an abnormal epidermis will cause many diseases. Phosphatase 2A (PP2A) is an important serine and threonine phosphatase. The α isoform of the PP2A catalytic subunit (Ppp2ca gene encoding PP2Acα) is critical for cell proliferation, growth, metabolism and tumorigenesis. However, to date, no study has revealed its roles in epidermis development. To specifically investigate the roles of PP2Acα in epidermis development, we first generated Ppp2caflox/flox transgenic mice, and conditionally knocked out Ppp2ca in the epidermis driven by Krt14-Cre. Our study showed that Ppp2caflox/flox; Krt14-Cre mice had significant hair loss. In addition, histological analyses showed that the morphogenesis and hair regeneration cycle of hair follicles were disrupted in these mice. Moreover, Ppp2caflox/flox; Krt14-Cre mice had smaller size, melanin deposition and hyperproliferation at the base of the claws. Accordingly, our study demonstrates that PP2Acα plays important roles in both hair follicle and epidermis development. Additionally, the Ppp2caflox/flox mice generated in this study can serve as a useful transgene model to study the roles of PP2Acα in other developmental processes and diseases. View Full-Text
Keywords: Ppp2ca; hair loss; Krt14-Cre; conditional knockout Ppp2ca; hair loss; Krt14-Cre; conditional knockout
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Fang, C.; Li, L.; Li, J. Conditional Knockout in Mice Reveals the Critical Roles of Ppp2ca in Epidermis Development. Int. J. Mol. Sci. 2016, 17, 756.

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