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Int. J. Mol. Sci. 2016, 17(5), 646; doi:10.3390/ijms17050646

Intense Resistance Exercise Promotes the Acute and Transient Nuclear Translocation of Small Ubiquitin-Related Modifier (SUMO)-1 in Human Myofibres

1
Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, 50933 Cologne, Germany
2
Institute for Dental Research and Oral Musculoskeletal Biology, University of Cologne, 50935 Cologne, Germany
3
Outpatient Clinic for Sports Traumatology and Public health Consultation, German Sport University Cologne, 50933 Cologne, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Julian Borejdo
Received: 22 March 2016 / Revised: 15 April 2016 / Accepted: 21 April 2016 / Published: 29 April 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Protein sumoylation is a posttranslational modification triggered by cellular stress. Because general information concerning the role of small ubiquitin-related modifier (SUMO) proteins in adult skeletal muscle is sparse, we investigated whether SUMO-1 proteins will be subjected to time-dependent changes in their subcellular localization in sarcoplasmic and nuclear compartments of human type I and II skeletal muscle fibers in response to acute stimulation by resistance exercise (RE). Skeletal muscle biopsies were taken at baseline (PRE), 15, 30, 60, 240 min and 24 h post RE from 6 male subjects subjected to a single bout of one-legged knee extensions. SUMO-1 localization was determined via immunohistochemistry and confocal laser microscopy. At baseline SUMO-1 was localized in perinuclear regions of myonuclei. Within 15 and up to 60 min post exercise, nuclear SUMO-1 localization was significantly increased (p < 0.01), declining towards baseline levels within 240 min post exercise. Sarcoplasmic SUMO-1 localization was increased at 15 min post exercise in type I and up to 30 min post RE in type II myofibres. The changing localization of SUMO-1 proteins acutely after intense muscle contractions points to a role for SUMO proteins in the acute regulation of the skeletal muscle proteome after exercise. View Full-Text
Keywords: SUMO-1; resistance exercise; sumoylation; skeletal muscle adaptation; nuclear translocation; acute response SUMO-1; resistance exercise; sumoylation; skeletal muscle adaptation; nuclear translocation; acute response
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gehlert, S.; Klinz, F.J.; Willkomm, L.; Schiffer, T.; Suhr, F.; Bloch, W. Intense Resistance Exercise Promotes the Acute and Transient Nuclear Translocation of Small Ubiquitin-Related Modifier (SUMO)-1 in Human Myofibres. Int. J. Mol. Sci. 2016, 17, 646.

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