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Int. J. Mol. Sci. 2016, 17(4), 556; doi:10.3390/ijms17040556

Prediction of Toxin Genes from Chinese Yellow Catfish Based on Transcriptomic and Proteomic Sequencing

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2,* , 1,2,* and 2,3,4,*
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Section on Marine biobank, China National Genebank, Shenzhen 518083, China
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BGI-Shenzhen, Shenzhen 518083, China
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Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China
4
Center for Marine Research, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Jun Li and Li Lin
Received: 14 March 2016 / Revised: 6 April 2016 / Accepted: 7 April 2016 / Published: 13 April 2016
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [7544 KB, uploaded 13 April 2016]   |  

Abstract

Fish venom remains a virtually untapped resource. There are so few fish toxin sequences for reference, which increases the difficulty to study toxins from venomous fish and to develop efficient and fast methods to dig out toxin genes or proteins. Here, we utilized Chinese yellow catfish (Pelteobagrus fulvidraco) as our research object, since it is a representative species in Siluriformes with its venom glands embedded in the pectoral and dorsal fins. In this study, we set up an in-house toxin database and a novel toxin-discovering protocol to dig out precise toxin genes by combination of transcriptomic and proteomic sequencing. Finally, we obtained 15 putative toxin proteins distributed in five groups, namely Veficolin, Ink toxin, Adamalysin, Za2G and CRISP toxin. It seems that we have developed a novel bioinformatics method, through which we could identify toxin proteins with high confidence. Meanwhile, these toxins can also be useful for comparative studies in other fish and development of potential drugs. View Full-Text
Keywords: Chinese yellow catfish; venom; transcriptome; proteome Chinese yellow catfish; venom; transcriptome; proteome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Xie, B.; Li, X.; Lin, Z.; Ruan, Z.; Wang, M.; Liu, J.; Tong, T.; Li, J.; Huang, Y.; Wen, B.; Sun, Y.; Shi, Q. Prediction of Toxin Genes from Chinese Yellow Catfish Based on Transcriptomic and Proteomic Sequencing. Int. J. Mol. Sci. 2016, 17, 556.

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