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Int. J. Mol. Sci. 2016, 17(4), 458; doi:10.3390/ijms17040458

Apolipoprotein A1-Unique Peptide as a Diagnostic Biomarker for Acute Ischemic Stroke

1
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China
2
Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, China
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 7 March 2016 / Revised: 17 March 2016 / Accepted: 22 March 2016 / Published: 28 March 2016
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Clinically-informative biomarkers of ischemic stroke are needed for rapid diagnosis and timely treatment. In the present study, APOA1 unique peptide (APOA1-UP), a novel peptide biomarker, was identified and quantified by multiple reaction monitoring (MRM) using labeled reference peptide (LRP). Serum samples of 94 patients in the ischemic stroke group and 37 patients in the non-stroke group were analyzed for the levels of total APOA1-UP, low density lipoprotein cholesterol (LDL-C), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and total cholesterol (TC). Median ratio of total APOA1-UP/LRP was 2.14 (interquartile range, 0.40) in the non-stroke group and 1.32 (0.44) in the ischemic stroke group (p < 0.0001). The serum level of total APOA1-UP was independently correlated with the presence of ischemic stroke by multivariate logistic regression analysis (p < 0.0001). From the receiver operating characteristic (ROC) curve, the area under the curve (AUC) was 0.9750 and the optimal cutoff value of the serum APOA1-UP level was 1.80, which yielded a sensitivity of 90.63% and a specificity of 97.14%. The diagnostic efficiency of HDL-C was lower, with an AUC of 0.7488. Therefore, the serum level of APOA1-UP is a diagnostic biomarker candidate for ischemic stroke in the early stage. View Full-Text
Keywords: apolipoprotein A1 unique peptide (APOA1-UP); acute ischemic stroke (AIS); biomarker; high-density lipoprotein cholesterol (HDL-C); multiple reaction monitoring (MRM); labeled reference peptide (LRP) apolipoprotein A1 unique peptide (APOA1-UP); acute ischemic stroke (AIS); biomarker; high-density lipoprotein cholesterol (HDL-C); multiple reaction monitoring (MRM); labeled reference peptide (LRP)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhao, X.; Yu, Y.; Xu, W.; Dong, L.; Wang, Y.; Gao, B.; Li, G.; Zhang, W. Apolipoprotein A1-Unique Peptide as a Diagnostic Biomarker for Acute Ischemic Stroke. Int. J. Mol. Sci. 2016, 17, 458.

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