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Int. J. Mol. Sci. 2016, 17(4), 435; doi:10.3390/ijms17040435

Knock-Down of Endogenous Bornavirus-Like Nucleoprotein 1 Inhibits Cell Growth and Induces Apoptosis in Human Oligodendroglia Cells

1,2,3,†
,
2,3,4,†
,
2,3,4,†
,
2,3
,
2,3
,
2,3
,
2,3
,
2,3
,
2,3
and
1,2,3,4,*
1
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, China
2
Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China
3
Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China
4
Department of Neurology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Alan C. Leonard
Received: 2 March 2016 / Revised: 16 March 2016 / Accepted: 18 March 2016 / Published: 24 March 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2834 KB, uploaded 24 March 2016]   |  

Abstract

Endogenous bornavirus-like nucleoprotein elements (EBLNs) have been discovered in the genomes of various animals including humans, whose functions have been seldom studied. To explore the biological functions of human EBLNs, we constructed a lentiviral vector expressing a short-hairpin RNA against human EBLN1, which successfully inhibited EBLN1 expression by above 80% in infected human oligodendroglia cells (OL cells). We found that EBLN1 silencing suppressed cell proliferation, induced G2/M phase arrest, and promoted apoptosis in OL cells. Gene expression profiling demonstrated that 1067 genes were up-regulated, and 2004 were down-regulated after EBLN1 silencing. The top 10 most upregulated genes were PI3, RND3, BLZF1, SOD2, EPGN, SBSN, INSIG1, OSMR, CREB3L2, and MSMO1, and the top 10 most-downregulated genes were KRTAP2-4, FLRT2, DIDO1, FAT4, ESCO2, ZNF804A, SUV420H1, ZC3H4, YAE1D1, and NCOA5. Pathway analysis revealed that these differentially expressed genes were mainly involved in pathways related to the cell cycle, the mitogen-activated protein kinase pathway, p53 signaling, and apoptosis. The gene expression profiles were validated by using quantitative reverse transcription polymerase chain reaction (RT-PCR) for detecting these 20 most-changed genes. Three genes closely related to glioma, RND3, OSMR, and CREB3L2, were significantly upregulated and might be the key factors in EBLN1 regulating the proliferation and apoptosis of OL cells. This study provides evidence that EBLN1 plays a key role in regulating cell life and death, thereby opening several avenues of investigation regarding EBLN1 in the future. View Full-Text
Keywords: endogenous bornavirus-like nucleoprotein 1; RNA interference; cDNA array; cell proliferation; apoptosis endogenous bornavirus-like nucleoprotein 1; RNA interference; cDNA array; cell proliferation; apoptosis
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MDPI and ACS Style

He, P.; Sun, L.; Zhu, D.; Zhang, H.; Zhang, L.; Guo, Y.; Liu, S.; Zhou, J.; Xu, X.; Xie, P. Knock-Down of Endogenous Bornavirus-Like Nucleoprotein 1 Inhibits Cell Growth and Induces Apoptosis in Human Oligodendroglia Cells. Int. J. Mol. Sci. 2016, 17, 435.

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