Genome Editing in C. elegans and Other Nematode Species
AbstractCaenorhabditis elegans, a 1 mm long free-living nematode, is a popular model animal that has been widely utilized for genetic investigations of various biological processes. Characteristic features that make C. elegans a powerful model of choice for eukaryotic genetic studies include its rapid life cycle (development from egg to adult in 3.5 days at 20 °C), well-annotated genome, simple morphology (comprising only 959 somatic cells in the hermaphrodite), and transparency (which facilitates non-invasive fluorescence observations). However, early approaches to introducing mutations in the C. elegans genome, such as chemical mutagenesis and imprecise excision of transposons, have required large-scale mutagenesis screens. To avoid this laborious and time-consuming procedure, genome editing technologies have been increasingly used in nematodes including C. briggsae and Pristionchus pacificus, thereby facilitating their genetic analyses. Here, I review the recent progress in genome editing technologies using zinc-finger nucleases (ZFNs), transcriptional activator-like nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 in nematodes and offer perspectives on their use in the future. View Full-Text
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Sugi, T. Genome Editing in C. elegans and Other Nematode Species. Int. J. Mol. Sci. 2016, 17, 295.
Sugi T. Genome Editing in C. elegans and Other Nematode Species. International Journal of Molecular Sciences. 2016; 17(3):295.Chicago/Turabian Style
Sugi, Takuma. 2016. "Genome Editing in C. elegans and Other Nematode Species." Int. J. Mol. Sci. 17, no. 3: 295.
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