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Int. J. Mol. Sci. 2016, 17(2), 236; doi:10.3390/ijms17020236

miR-9-5p, miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation, Differentiation, and Adhesion

1
Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
2
Shenzhen Key Laboratory for Innovative Technology, The University of Hong Kong Shenzhen Hospital, Shenzhen 518000, China
3
Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518000, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Y-h. Taguchi
Received: 1 January 2016 / Revised: 4 February 2016 / Accepted: 5 February 2016 / Published: 15 February 2016
(This article belongs to the Special Issue MicroRNA Regulation)
View Full-Text   |   Download PDF [1998 KB, uploaded 15 February 2016]   |  

Abstract

This study was designed to evaluate the effects of strontium on the expression levels of microRNAs (miRNAs) and to explore their effects on skeletal cell proliferation, differentiation, adhesion, and apoptosis. The targets of these miRNAs were also studied. Molecular cloning, cell proliferation assay, cell apoptosis assay, quantitative real-time PCR, and luciferase reporter assay were used. Strontium altered the expression levels of miRNAs in vitro and in vivo. miR-9-5p, miR-675-5p, and miR-138-5p impaired skeletal cell proliferation, cell differentiation and cell adhesion. miR-9-5p and miR-675-5p induced MC3T3-E1 cell apoptosis more specifically than miR-138-5p. miR-9-5p, miR-675-5p, and miR-138-5p targeted glycogen synthase kinase 3 β (GSK3β), ATPase Aminophospholipid Transporter Class I Type 8A Member 2 (ATP8A2), and Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1), respectively. Low-density lipoprotein receptor-related protein 5 (LRP5) played a positive role in skeletal development. miR-9-5p, miR-675-5p, and miR-138-5p damage strontium and LRP5-mediated skeletal cell proliferation, differentiation, and adhesion, and induce cell apoptosis by targeting GSK3β, ATP8A2, and EIF4EBP1, respectively. View Full-Text
Keywords: microRNA or miRNA; bone; strontium; cell proliferation; differentiation; adhesion and apoptosis; target; LRP5 microRNA or miRNA; bone; strontium; cell proliferation; differentiation; adhesion and apoptosis; target; LRP5
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sun, T.; Leung, F.; Lu, W.W. miR-9-5p, miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation, Differentiation, and Adhesion. Int. J. Mol. Sci. 2016, 17, 236.

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