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Int. J. Mol. Sci. 2016, 17(2), 204; doi:10.3390/ijms17020204

Isolation and Characterization of a Novel Dicistrovirus Associated with Moralities of the Great Freshwater Prawn, Macrobrachium rosenbergii

1
Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China
2
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
3
State key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan 430071, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Jun Li and Li Lin
Received: 4 December 2015 / Revised: 20 January 2016 / Accepted: 26 January 2016 / Published: 2 February 2016
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [1857 KB, uploaded 2 February 2016]   |  

Abstract

The giant freshwater prawn, Macrobrachium rosenbergii, is an economically important crustacean and is farmed in many countries. Since 2009, a larval mortality syndrome of M. rosenbergii has broken out and spread widely in the main breeding area, including Zhejiang, Jiangsu, Guangxi, and Guangdong Provinces in mainland China. A novel virus, named Macrobrachium rosenbergii Taihu virus (MrTV), was isolated from the moribund larvae and was determined to be the causative agent of the M. rosenbergii larval mortality syndrome by experimental infection. Further genomic sequencing suggested that the MrTV genome is monopartite, 10,303 nt in length, and dicistronic with two non-overlapping open reading frames (ORFs) separated by an intergenic region (IGR) and flanked by untranslated regions (UTRs). Phylogenetic analysis using the full-length genomic sequence and the putative amino acid sequences of the capsid protein revealed that MrTV was more closely related to the taura syndrome virus (TSV) than to any other viruses. According to these molecular features, we proposed that MrTV is a new species in the genus Aparavirus, family Dicistroviridae. These results may shed light on controlling larval mortality syndrome in M. rosenbergii. View Full-Text
Keywords: Macrobrachium rosenbergii; dicistrovirus; Aparavirus; taihu virus; larval mortality syndrome Macrobrachium rosenbergii; dicistrovirus; Aparavirus; taihu virus; larval mortality syndrome
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MDPI and ACS Style

Pan, X.; Cao, Z.; Yuan, J.; Shi, Z.; Yuan, X.; Lin, L.; Xu, Y.; Yao, J.; Hao, G.; Shen, J. Isolation and Characterization of a Novel Dicistrovirus Associated with Moralities of the Great Freshwater Prawn, Macrobrachium rosenbergii. Int. J. Mol. Sci. 2016, 17, 204.

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