Next Article in Journal
Review of Toxic Epidermal Necrolysis
Next Article in Special Issue
Triptolide Combined with Radiotherapy for the Treatment of Nasopharyngeal Carcinoma via NF-κB-Related Mechanism
Previous Article in Journal
Modulation of GLO1 Expression Affects Malignant Properties of Cells
Previous Article in Special Issue
Development of Cell-SELEX Technology and Its Application in Cancer Diagnosis and Therapy
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2016, 17(12), 2134; doi:10.3390/ijms17122134

Recent Methods for Purification and Structure Determination of Oligonucleotides

1,3,4,†
,
1,3,4,†
,
1,3,4
,
1,3,4
,
1,3,4
,
1,3,4
,
1,3,4
,
1,2,3,4,* , 1,3,4,* and 1,3,4,*
1
Institute of Integrated Bioinformedicine and Translational Science, School of Chinese Medicine, Hong Kong Baptist University (HKBU), Hong Kong, China
2
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China
3
Institute of Precision Medicine and Innovative Drug Discovery, HKBU (Haimen) Institute of Science and Technology, Haimen 226100, China
4
Shenzhen Lab of Combinatorial Compounds and Targeted Drug Delivery, HKBU Institute of Research and Continuing Education, Shenzhen 518000, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Mateus Webba da Silva
Received: 25 November 2016 / Revised: 13 December 2016 / Accepted: 14 December 2016 / Published: 18 December 2016
(This article belongs to the Special Issue Translational Molecular Medicine & Molecular Drug Discovery)
View Full-Text   |   Download PDF [570 KB, uploaded 21 December 2016]   |  

Abstract

Aptamers are single-stranded DNA or RNA oligonucleotides that can interact with target molecules through specific three-dimensional structures. The excellent features, such as high specificity and affinity for target proteins, small size, chemical stability, low immunogenicity, facile chemical synthesis, versatility in structural design and engineering, and accessible for site-specific modifications with functional moieties, make aptamers attractive molecules in the fields of clinical diagnostics and biopharmaceutical therapeutics. However, difficulties in purification and structural identification of aptamers remain a major impediment to their broad clinical application. In this mini-review, we present the recently attractive developments regarding the purification and identification of aptamers. We also discuss the advantages, limitations, and prospects for the major methods applied in purifying and identifying aptamers, which could facilitate the application of aptamers. View Full-Text
Keywords: aptamers; purification; identification aptamers; purification; identification
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhang, Q.; Lv, H.; Wang, L.; Chen, M.; Li, F.; Liang, C.; Yu, Y.; Jiang, F.; Lu, A.; Zhang, G. Recent Methods for Purification and Structure Determination of Oligonucleotides. Int. J. Mol. Sci. 2016, 17, 2134.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top