Next Article in Journal
Recent Methods for Purification and Structure Determination of Oligonucleotides
Next Article in Special Issue
Bacterial Responses to Glyoxal and Methylglyoxal: Reactive Electrophilic Species
Previous Article in Journal
EGFR and KRAS Mutations Predict the Incidence and Outcome of Brain Metastases in Non-Small Cell Lung Cancer
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(12), 2133; doi:10.3390/ijms17122133

Modulation of GLO1 Expression Affects Malignant Properties of Cells

1
Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, Leipzig 04103, Germany
2
Max Planck Institute of Evolutionary Anthropology, Deutscher Platz 6, Leipzig 04103, Germany
3
Institute of Medical Microbiology, Faculty of Medicine, University of Leipzig, Liebigstrasse 21, Leipzig 04103, Germany
4
College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar P.O. Box 79, Ethiopia
5
Department of Neurosurgery, University Hospital Leipzig, Liebigstrasse 20, Leipzig 04103, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Casper Schalkwijk
Received: 31 August 2016 / Revised: 8 December 2016 / Accepted: 12 December 2016 / Published: 18 December 2016
(This article belongs to the Special Issue Glyoxalase System)
View Full-Text   |   Download PDF [2256 KB, uploaded 19 December 2016]   |  

Abstract

The energy metabolism of most tumor cells relies on aerobic glycolysis (Warburg effect) characterized by an increased glycolytic flux that is accompanied by the increased formation of the cytotoxic metabolite methylglyoxal (MGO). Consequently, the rate of detoxification of this reactive glycolytic byproduct needs to be increased in order to prevent deleterious effects to the cells. This is brought about by an increased expression of glyoxalase 1 (GLO1) that is the rate-limiting enzyme of the MGO-detoxifying glyoxalase system. Here, we overexpressed GLO1 in HEK 293 cells and silenced it in MCF-7 cells using shRNA. Tumor-related properties of wild type and transformed cells were compared and key glycolytic enzyme activities assessed. Furthermore, the cells were subjected to hypoxic conditions to analyze the impact on cell proliferation and enzyme activities. Our results demonstrate that knockdown of GLO1 in the cancer cells significantly reduced tumor-associated properties such as migration and proliferation, whereas no functional alterations where found by overexpression of GLO1 in HEK 293 cells. In contrast, hypoxia caused inhibition of cell growth of all cells except of those overexpressing GLO1. Altogether, we conclude that GLO1 on one hand is crucial to maintaining tumor characteristics of malignant cells, and, on the other hand, supports malignant transformation of cells in a hypoxic environment when overexpressed. View Full-Text
Keywords: glyoxalase 1; MCF-7 cells; HEK 293 cell; aerobic glycolysis; malignant transformation; methylglyoxal glyoxalase 1; MCF-7 cells; HEK 293 cell; aerobic glycolysis; malignant transformation; methylglyoxal
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hutschenreuther, A.; Bigl, M.; Hemdan, N.Y.A.; Debebe, T.; Gaunitz, F.; Birkenmeier, G. Modulation of GLO1 Expression Affects Malignant Properties of Cells. Int. J. Mol. Sci. 2016, 17, 2133.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top