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Int. J. Mol. Sci. 2016, 17(12), 2092; doi:10.3390/ijms17122092

Zinc Up-Regulates Insulin Secretion from β Cell-Like Cells Derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED)

1
School of Medicine Department of Obstetrics, Gynecology and Reproductive Sciences, University of Maryland, Baltimore, MD 21201, USA
2
UCLA School of Dentistry, Los Angeles, CA 90095, USA
3
Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Toshiyuki Fukada
Received: 29 September 2016 / Revised: 1 December 2016 / Accepted: 6 December 2016 / Published: 13 December 2016
(This article belongs to the Special Issue Zinc Signaling in Physiology and Pathogenesis)
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Abstract

Stem cells from human exfoliated deciduous tooth (SHED) offer several advantages over other stem cell sources. Using SHED, we examined the roles of zinc and the zinc uptake transporter ZIP8 (Zrt- and irt-like protein 8) while inducing SHED into insulin secreting β cell-like stem cells (i.e., SHED-β cells). We observed that ZIP8 expression increased as SHED differentiated into SHED-β cells, and that zinc supplementation at day 10 increased the levels of most pancreatic β cell markers—particularly Insulin and glucose transporter 2 (GLUT2). We confirmed that SHED-β cells produce insulin successfully. In addition, we note that zinc supplementation significantly increases insulin secretion with a significant elevation of ZIP8 transporters in SHED-β cells. We conclude that SHED can be converted into insulin-secreting β cell-like cells as zinc concentration in the cytosol is elevated. Insulin production by SHED-β cells can be regulated via modulation of zinc concentration in the media as ZIP8 expression in the SHED-β cells increases. View Full-Text
Keywords: dental pulp; insulin; stem cells; zinc; Zrt- and irt-like protein (ZIP) dental pulp; insulin; stem cells; zinc; Zrt- and irt-like protein (ZIP)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, G.; Shin, K.-H.; Pae, E.-K. Zinc Up-Regulates Insulin Secretion from β Cell-Like Cells Derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED). Int. J. Mol. Sci. 2016, 17, 2092.

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